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验证数据展示

  • IHC staining of human colon using 27794-1-AP

    IHC staining of human colon using 27794-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27794-1-AP (Periostin antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Periostin antibody included
  • IHC staining of human colon using 27794-1-AP

    IHC staining of human colon using 27794-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27794-1-AP (Periostin antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Periostin antibody included

Periostin Polyclonal antibody

Periostin Polyclonal Antibody for IHC, ELISA
Cat No. 27794-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Synonyms

PDLPOSTN, Osteoblast-specific factor 2, Osteoblast specific factor 2, OSF-2, OSF2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IHC detected inhuman colon tissue, human colon cancer tissue, human breast cancer tissue, human liver cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy Periostin Ready-To-Use IHC Kit. Periostin primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27794-1-AP targets Periostin in IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, ELISA Application Description
Tested Reactivity human
Immunogen Periostin fusion protein Ag27145 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name periostin, osteoblast specific factor
Synonyms PDLPOSTN, Osteoblast-specific factor 2, Osteoblast specific factor 2, OSF-2, OSF2
GenBank Accession NumberBC106710
Gene Symbol Periostin
Gene ID (NCBI) 10631
RRIDAB_2880975
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15063
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Periostin (POSTN, PN), originally named as osteoblast-specific factor 2 (OSF-2), is a 90-kDa secreted protein which is now classified as a matricellular protein. It is present in a wide variety of normal adult tissues and fetal tissues, and has a role in bone, tooth and heart development and function. Studies show that periostin is overexpressed in a broad range of human cancer types, including lung, ovary, breast and colon cancers. Recent evidence reveals that periostin is expressed by fibroblasts in the normal tissue and in the stroma of the primary tumour, and it is required to allow cancer stem cell maintenance.

实验方案

Product Specific Protocols
IHC protocol for Periostin antibody 27794-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols