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验证数据展示

  • WB analysis of BxPC-3 using 66491-1-Ig (same clone as 66491-1-PBS)

    WB analysis of BxPC-3 using 66491-1-Ig (same clone as 66491-1-PBS)

    BxPC-3, SGC-7901 and ROS1728 cells were subjected to SDS PAGE followed by western blot with 66491-1-Ig (Periostin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66491-1-Ig (same clone as 66491-1-PBS)

    IHC staining of human breast cancer using 66491-1-Ig (same clone as 66491-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66491-1-Ig (Periostin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Periostin antibody included
  • IHC staining of human breast cancer using 66491-1-Ig (same clone as 66491-1-PBS)

    IHC staining of human breast cancer using 66491-1-Ig (same clone as 66491-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66491-1-Ig (Periostin antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Periostin antibody included
  • IHC staining of human colon using 66491-1-Ig (same clone as 66491-1-PBS)

    IHC staining of human colon using 66491-1-Ig (same clone as 66491-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66491-1-Ig (Periostin antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Periostin antibody included
  • IHC staining of human stomach using 66491-1-Ig (same clone as 66491-1-PBS)

    IHC staining of human stomach using 66491-1-Ig (same clone as 66491-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66491-1-Ig (Periostin antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Periostin antibody included
  • IF Staining of human breast cancer using 66491-1-Ig (same clone as 66491-1-PBS)

    IF Staining of human breast cancer using 66491-1-Ig (same clone as 66491-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Periostin antibody (66491-1-Ig, Clone: 1A11A3 ) at dilution of 1:8000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 66491-1-Ig (same clone as 66491-1-PBS)

    IF Staining of human breast cancer using 66491-1-Ig (same clone as 66491-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Periostin antibody (66491-1-Ig, Clone: 1A11A3 ) at dilution of 1:8000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse breast cancer using 66491-1-Ig (same clone as 66491-1-PBS)

    IF Staining of mouse breast cancer using 66491-1-Ig (same clone as 66491-1-PBS)

    Immunofluorescent analysis of un-fixed frozen OCT-embedded mouse breast cancer using Periostin antibody (66491-1-Ig, Clone: 1A11A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Periostin Monoclonal antibody, PBS Only

Periostin Monoclonal Antibody for WB, IHC, IF-P, IF-Fro, ELISA
Cat No. 66491-1-PBS

产品说明书

CloneNo. 1A11A3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IF-Fro, ELISA

别名

1A11A3, OSF 2, OSF2, OSF-2, Osteoblast specific factor 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy Periostin Ready-To-Use IHC Kit. Periostin primary antibody included.

产品信息

66491-1-PBS targets Periostin in WB, IHC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IF-Fro, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Periostin fusion protein Ag14487 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 periostin, osteoblast specific factor
别名 1A11A3, OSF 2, OSF2, OSF-2, Osteoblast specific factor 2
计算分子量 93 kDa
观测分子量 85-90 kDa
GenBank蛋白编号BC106710
基因名称 Periostin
Gene ID (NCBI) 10631
RRIDAB_2881856
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ15063
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Periostin (POSTN, PN), originally named as osteoblast-specific factor 2 (OSF-2), is a 90-kDa secreted protein which is now classified as a matricellular protein. It is present in a wide variety of normal adult tissues and fetal tissues, and has a role in bone, tooth and heart development and function. Studies show that periostin is overexpressed in a broad range of human cancer types, including lung, ovary, breast and colon cancers. Recent evidence reveals that periostin is expressed by fibroblasts in the normal tissue and in the stroma of the primary tumour, and it is required to allow cancer stem cell maintenance. The isoforms of periostin are between 83 and 93 kDa in mass and differ in their C-terminal sequences, characterized by individual presence or absence of cassette exons 17-21 (UniProtKB/Swiss-Prot, PMID: 21997759).