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  • Featured Product
  • KD/KO Validated

Periostin Monoclonal antibody, PBS Only

Periostin Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1A11A3

Cat no : 66491-1-PBS

Print datasheet

Synonyms

Osteoblast-specific factor 2, Osteoblast specific factor 2, OSF-2, OSF2, OSF 2

验证数据展示

  • WB analysis of BxPC-3 using 66491-1-Ig (same clone as 66491-1-PBS)

    WB analysis of BxPC-3 using 66491-1-Ig (same clone as 66491-1-PBS)

    BxPC-3, SGC-7901 and ROS1728 cells were subjected to SDS PAGE followed by western blot with 66491-1-Ig (Periostin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 66491-1-Ig (same clone as 66491-1-PBS)

    IHC staining of human colon using 66491-1-Ig (same clone as 66491-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66491-1-Ig (Periostin antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Periostin antibody included
  • IHC staining of human breast cancer using 66491-1-Ig (same clone as 66491-1-PBS)

    IHC staining of human breast cancer using 66491-1-Ig (same clone as 66491-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66491-1-Ig (Periostin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Periostin antibody included
  • IHC staining of human breast cancer using 66491-1-Ig (same clone as 66491-1-PBS)

    IHC staining of human breast cancer using 66491-1-Ig (same clone as 66491-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66491-1-Ig (Periostin antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Periostin antibody included
  • IF Staining of human breast cancer using 66491-1-Ig (same clone as 66491-1-PBS)

    IF Staining of human breast cancer using 66491-1-Ig (same clone as 66491-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Periostin antibody (66491-1-Ig, Clone: 1A11A3 ) at dilution of 1:8000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 66491-1-Ig (same clone as 66491-1-PBS)

    IF Staining of human breast cancer using 66491-1-Ig (same clone as 66491-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Periostin antibody (66491-1-Ig, Clone: 1A11A3 ) at dilution of 1:8000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66491-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy Periostin Ready-To-Use IHC Kit. Periostin primary antibody included.

产品信息

66491-1-PBS targets Periostin in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Periostin fusion protein Ag14487 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name periostin, osteoblast specific factor
Synonyms Osteoblast-specific factor 2, Osteoblast specific factor 2, OSF-2, OSF2, OSF 2
Calculated Molecular Weight 93 kDa
Observed Molecular Weight 85-90 kDa
GenBank Accession NumberBC106710
Gene Symbol Periostin
Gene ID (NCBI) 10631
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15063
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Periostin (POSTN, PN), originally named as osteoblast-specific factor 2 (OSF-2), is a 90-kDa secreted protein which is now classified as a matricellular protein. It is present in a wide variety of normal adult tissues and fetal tissues, and has a role in bone, tooth and heart development and function. Studies show that periostin is overexpressed in a broad range of human cancer types, including lung, ovary, breast and colon cancers. Recent evidence reveals that periostin is expressed by fibroblasts in the normal tissue and in the stroma of the primary tumour, and it is required to allow cancer stem cell maintenance. The isoforms of periostin are between 83 and 93 kDa in mass and differ in their C-terminal sequences, characterized by individual presence or absence of cassette exons 17-21 (UniProtKB/Swiss-Prot, PMID: 21997759).