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Peripherin Monoclonal antibody

Peripherin Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat, pig

Applications

FC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H10B3

Cat No : 66317-1-Ig

Print datasheet

Synonyms

NEF4, Neurofilament 4, Peripherin, PRPH, PRPH1

验证数据展示

  • FC experiment of PC-12 using 66317-1-Ig

    FC experiment of PC-12 using 66317-1-Ig

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human Peripherin (66317-1-Ig, Clone:1H10B3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis of rat brain using 66317-1-Ig

    WB analysis of rat brain using 66317-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66317-1-Ig (Peripherin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 66317-1-Ig

    IHC staining of human brain using 66317-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66317-1-Ig (Peripherin antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 66317-1-Ig

    IHC staining of human brain using 66317-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66317-1-Ig (Peripherin antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human colon using 66317-1-Ig

    IHC staining of human colon using 66317-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66317-1-Ig (Peripherin Antibody) at dilution of 1:1000 (under 40x lens).

  • FC experiment of SH-SY5Y using 66317-1-Ig

    FC experiment of SH-SY5Y using 66317-1-Ig

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human Peripherin (66317-1-Ig, Clone:1H10B3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inrat brain tissue, human colon tissue, rat colon tissue
Positive IHC detected inhuman brain tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inPC-12 cells, SH-SY5Y cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66317-1-Ig targets Peripherin in WB, FC, IHC, ELISA applications and shows reactivity with human, rat, pig samples.

Tested Applications FC, IHC, WB, ELISA
Tested Reactivity human, rat, pig
Immunogen Peripherin fusion protein Ag11116 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name peripherin
Synonyms NEF4, Neurofilament 4, Peripherin, PRPH, PRPH1
Calculated Molecular Weight 470 aa, 54 kDa
Observed Molecular Weight 50-54 kDa
GenBank Accession NumberBC032703
Gene Symbol PRPH
Gene ID (NCBI) 5630
RRIDAB_2881698
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP41219
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Peripherin antibody 66317-1-IgDownload protocol
IHC protocol for Peripherin antibody 66317-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols