Phospho-AKT (Ser473) Monoclonal antibody, PBS Only (Capture)

Phospho-AKT (Ser473) Monoclonal Antibody for WB, IHC, Indirect ELISA, Cytometric bead array

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

1C10B8

Cat No : 66444-1-PBS

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Synonyms

AKT, AKT1, pakt, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha



Planning an IHC experiment? We recommend our IHCeasy AKT Ready-To-Use IHC Kit. AKT primary antibody included.

产品信息

66444-1-PBS targets Phospho-AKT (Ser473) as part of a matched antibody pair:

MP50177-1: 66444-1-PBS capture and 60203-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, Indirect ELISA, Cytometric bead array Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name v-akt murine thymoma viral oncogene homolog 1
Synonyms AKT, AKT1, pakt, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha
Observed Molecular Weight 60-62 kDa
GenBank Accession NumberNM_005163
Gene Symbol AKT1
Gene ID (NCBI) 207
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP31749
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. This antibody detects all the members of AKT with phospho-modification at Ser473.