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Phospho-ALK (Tyr1604) Polyclonal antibody

Phospho-ALK (Tyr1604) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30266-1-AP

Print datasheet

Synonyms

ALK, ALK tyrosine kinase receptor, Anaplastic lymphoma kinase, CD246, Phospho-ALK (Tyr1604), TFG/ALK

验证数据展示

  • WB analysis using 30266-1-AP

    WB analysis using 30266-1-AP

    Non-treated and PMA treated HL-60 cells were subjected to SDS PAGE followed by western blot with 30266-1-AP (Phospho-ALK (Tyr1604) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (66305-1-Ig) antibody as loading control.

经过测试的应用

Positive WB detected inPMA treated HL-60 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30266-1-AP targets Phospho-ALK (Tyr1604) in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name anaplastic lymphoma receptor tyrosine kinase
Synonyms ALK, ALK tyrosine kinase receptor, Anaplastic lymphoma kinase, CD246, Phospho-ALK (Tyr1604), TFG/ALK
Observed Molecular Weight140 kDa
GenBank Accession NumberBC157090
Gene Symbol ALK
Gene ID (NCBI) 238
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UM73
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ALK, also named as CD246, is a receptor tyrosine kinase (RTK) that belongs to the protein kinase superfamily. ALK is usually found in the nervous system and appears to play an important role in the normal development and function of the nervous system. ALK was originally identified as part of the NPM (Nucleophosmin)-ALK oncogenic fusion protein, resulting from the (2;5)(p23;q35) translocation that is frequently associated with anaplastic large-cell lymphoma (ALCL). The human ALK appears to exist as a 140 kDa protein, as well as the 220 kDa full-length ALK species and the 140 kDa ALK species is thought to be the result of a cleavage within the extracellular region of full-length ALK. The 140 kDa ALK protein is phosphorylated in response to activation of ALK(PMID: 19459784).

实验方案

Product Specific Protocols
WB protocol for Phospho-ALK (Tyr1604) antibody 30266-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols