验证数据展示
经过测试的应用
| Positive WB detected in | PMA treated HL-60 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
30266-1-AP targets Phospho-ALK (Tyr1604) in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human |
| 文献引用反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | anaplastic lymphoma receptor tyrosine kinase |
| 别名 | ALK, ALK tyrosine kinase receptor, Anaplastic lymphoma kinase, CD246, Phospho-ALK (Tyr1604), TFG/ALK |
| 观测分子量 | 140 kDa |
| GenBank蛋白编号 | BC157090 |
| 基因名称 | ALK |
| Gene ID (NCBI) | 238 |
| RRID | AB_2935424 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UM73 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ALK, also named as CD246, is a receptor tyrosine kinase (RTK) that belongs to the protein kinase superfamily. ALK is usually found in the nervous system and appears to play an important role in the normal development and function of the nervous system. ALK was originally identified as part of the NPM (Nucleophosmin)-ALK oncogenic fusion protein, resulting from the (2;5)(p23;q35) translocation that is frequently associated with anaplastic large-cell lymphoma (ALCL). The human ALK appears to exist as a 140 kDa protein, as well as the 220 kDa full-length ALK species and the 140 kDa ALK species is thought to be the result of a cleavage within the extracellular region of full-length ALK. The 140 kDa ALK protein is phosphorylated in response to activation of ALK(PMID: 19459784).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-ALK (Tyr1604) antibody 30266-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |