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验证数据展示

  • WB analysis using 28847-1-AP

    WB analysis using 28847-1-AP

    Phosphatase inhibitor treated and λ phosphatase treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 28847-1-AP (Phospho-ASK1 (Ser83) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Phospho-ASK1 (Ser83) Polyclonal antibody

Phospho-ASK1 (Ser83) Polyclonal Antibody for WB, ELISA
Cat No. 28847-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

ASK 1, ASK1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, MEK kinase 5, MEKK 5, MEKK5, Phospho-ASK1 (Ser83)

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, λ phosphatase treated HEK-293T cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28847-1-AP targets Phospho-ASK1 (Ser83) in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitogen-activated protein kinase kinase kinase 5
Synonyms ASK 1, ASK1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, MEK kinase 5, MEKK 5, MEKK5, Phospho-ASK1 (Ser83)
Calculated Molecular Weight 155 kDa
Observed Molecular Weight150 kDa
GenBank Accession NumberBC054503
Gene Symbol ASK1
Gene ID (NCBI) 4217
RRIDAB_3085337
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99683
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ASK1(Apoptosis signal-regulating kinase 1) is also named as MAP3K5, MAPKKK5, MEKK5 and belongs to the MAP kinase kinase kinase subfamily. ASK1 is reportedly regulated by phosphorylation and dephosphorylation at four different phosphorylation sites. ASK1 activation is accompanied by phosphorylation of Thr845 and dephosphorylation of Ser83, Ser967, and Ser1034. STRAP actually decreased Thr838 (corresponding to Thr845 in mice) phosphorylation but increased Ser83 and Ser967 phosphorylation, an effect that would inactivate ASK1. (PMID: 19880523)

实验方案

Product Specific Protocols
WB protocol for Phospho-ASK1 (Ser83) antibody 28847-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols