验证数据展示
经过测试的应用
Positive WB detected in | Anisomycin treated NIH/3T3 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:16000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
28790-1-AP targets Phospho-ATF2 (Thr71)/ATF7 (Thr53) in WB, ELISA applications and shows reactivity with Human, Mouse samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | Human, Mouse |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | activating transcription factor 2 |
别名 | ATF2, ATF7, CRE BP1, CREB 2, CREB2, CREBP1, HB16, Phospho-ATF2 (Thr71), Phospho-ATF2 (Thr71)/ATF7 (Thr53), Phospho-ATF7 (Thr53), TREB7 |
计算分子量 | 209 aa, 23 kDa |
观测分子量 | 60-70 kDa |
GenBank蛋白编号 | BC026175 |
基因名称 | ATF2 |
Gene ID (NCBI) | 1386 |
RRID | AB_3085335 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P15336 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATF2, also named as CREB2 and CREBP1, contains one bZIP domain and one C2H2-type zinc finger. It belongs to the bZIP family. ATF2 binds to the cAMP-responsive element(CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. ATF2 binds DNA as a dimer and can form a homodimer in the absence of DNA. It binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity. Stress and growth factors activate ATF2 and ATF7 mainly via sequential phosphorylation of two conserved threonine residues in their activation domain. Distinct protein kinases, among which mitogen-activated protein kinases (MAPK), phosphorylate ATF2 on Thr71 and ATF7 on Thr53, resulting in transcriptional activation. The antibody recognizes ATF2 phosphorylation sites Thr71 and ATF7 phosphorylation sites Thr53.
实验方案
Product Specific Protocols | |
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WB protocol for Phospho-ATF2 (Thr71)/ATF7 (Thr53) antibody 28790-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |