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验证数据展示

  • WB analysis using 28790-1-AP

    WB analysis using 28790-1-AP

    Non-treated NIH/3T3 cells and Anisomycin treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28790-1-AP (Phospho-ATF2 (Thr71)/ATF7 (Thr53) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Phospho-ATF2 (Thr71)/ATF7 (Thr53) Polyclonal antibody

Phospho-ATF2 (Thr71)/ATF7 (Thr53) Polyclonal Antibody for WB, ELISA
Cat No. 28790-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, ELISA

Synonyms

ATF2, ATF7, CRE BP1, CREB 2, CREB2, CREBP1, HB16, Phospho-ATF2 (Thr71), Phospho-ATF2 (Thr71)/ATF7 (Thr53), Phospho-ATF7 (Thr53), TREB7

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inAnisomycin treated NIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28790-1-AP targets Phospho-ATF2 (Thr71)/ATF7 (Thr53) in WB, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name activating transcription factor 2
Synonyms ATF2, ATF7, CRE BP1, CREB 2, CREB2, CREBP1, HB16, Phospho-ATF2 (Thr71), Phospho-ATF2 (Thr71)/ATF7 (Thr53), Phospho-ATF7 (Thr53), TREB7
Calculated Molecular Weight 209 aa, 23 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC026175
Gene Symbol ATF2
Gene ID (NCBI) 1386
RRIDAB_3085335
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP15336
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATF2, also named as CREB2 and CREBP1, contains one bZIP domain and one C2H2-type zinc finger. It belongs to the bZIP family. ATF2 binds to the cAMP-responsive element(CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. ATF2 binds DNA as a dimer and can form a homodimer in the absence of DNA. It binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity. Stress and growth factors activate ATF2 and ATF7 mainly via sequential phosphorylation of two conserved threonine residues in their activation domain. Distinct protein kinases, among which mitogen-activated protein kinases (MAPK), phosphorylate ATF2 on Thr71 and ATF7 on Thr53, resulting in transcriptional activation. The antibody recognizes ATF2 phosphorylation sites Thr71 and ATF7 phosphorylation sites Thr53.

实验方案

Product Specific Protocols
WB protocol for Phospho-ATF2 (Thr71)/ATF7 (Thr53) antibody 28790-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols