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Phospho-Beta Catenin (Ser552) Polyclonal antibody

Phospho-Beta Catenin (Ser552) Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28778-1-AP

Print datasheet

Synonyms

b cat, Beta catenin, Catenin beta 1, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, Phospho-Beta Catenin (Ser552), Phospho-CTNNB1 (Ser552), PRO2286, β-Catenin

验证数据展示

  • WB analysis using 28778-1-AP

    WB analysis using 28778-1-AP

    Untreated and Forskolin treated cells were subjected to SDS PAGE followed by western blot with 28778-1-AP (Phospho-Beta Catenin (Ser552) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as loading control.

  • IF Staining of MCF-7 using 28778-1-AP

    IF Staining of MCF-7 using 28778-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Phospho-Beta Catenin (Ser552) antibody (28778-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inForskolin treated A431 cells, HSC-T6 cells, Forskolin treated HSC-T6 cells, HT-29 cells, Forskolin treated HT-29 cells
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy Beta Catenin Ready-To-Use IHC Kit. Beta Catenin primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28778-1-AP targets Phospho-Beta Catenin (Ser552) in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name catenin (cadherin-associated protein), beta 1, 88kDa
Synonyms b cat, Beta catenin, Catenin beta 1, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, Phospho-Beta Catenin (Ser552), Phospho-CTNNB1 (Ser552), PRO2286, β-Catenin
Calculated Molecular Weight 781 aa, 86 kDa
Observed Molecular Weight86-100 kDa
GenBank Accession NumberBC058926
Gene Symbol Beta Catenin
Gene ID (NCBI) 1499
ENSEMBL Gene IDENSG00000168036
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35222
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin is a 92-kDa protein, originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers(PMID: 22617422, PMID: 18334222). Both AMPK and AKT phosphorylates β-catenin at Ser 552. The phosphorylation of β-catenin at Ser552 can stabilize β-catenin, enhance its nuclear accumulation and increase its transcriptional activity(PMID: 2865606, PMID: 20361929).

实验方案

Product Specific Protocols
WB protocol for Phospho-Beta Catenin (Ser552) antibody 28778-1-APDownload protocol
IF protocol for Phospho-Beta Catenin (Ser552) antibody 28778-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols