Phospho-Beta Catenin (Ser675) Polyclonal antibody
Phospho-Beta Catenin (Ser675) Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human and More (3)
Applications
WB, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | λ phosphatase treated HT-29 cells |
| Positive IF/ICC detected in | λ phosphatase treated MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy Beta Catenin Ready-To-Use IHC Kit. Beta Catenin primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 9 publications below |
| IHC | See 1 publications below |
产品信息
28853-1-AP targets Phospho-Beta Catenin (Ser675) in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse, bovine |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | catenin (cadherin-associated protein), beta 1, 88kDa |
| Synonyms | b cat, Beta catenin, Catenin beta 1, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, Phospho-Beta Catenin (Ser675), PRO2286, β-Catenin |
| Calculated Molecular Weight | 781 aa, 86 kDa |
| Observed Molecular Weight | 90-100 kDa |
| GenBank Accession Number | BC058926 |
| Gene Symbol | Beta Catenin |
| Gene ID (NCBI) | 1499 |
| ENSEMBL Gene ID | ENSG00000168036 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P35222 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Beta Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. Beta catenin is a core component of the cadherin protein complex, whose stabilization is essential for the activation of Wnt/β-catenin signaling. Phosphorylation of Ser675 in the C-terminus of at beta catenin promotesmits nuclear accumulation and transcriptional activity, resulting in increased activation of the Wnt/β-catenin signaling pathway(PMID: 25980439). In addition, Ser675 phosphorylation promotes beta catenin interaction with the transcriptional coactivator CREB-binding protein in the nucleus(PMID: 16476742).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-Beta Catenin (Ser675) antibody 28853-1-AP | Download protocol |
| IF protocol for Phospho-Beta Catenin (Ser675) antibody 28853-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Endocrinology Casein kinase 1α regulates testosterone synthesis and testis development in adult mice | ||
FASEB J Verteporfin attenuates trauma-induced heterotopic ossification of Achilles tendon by inhibiting osteogenesis and angiogenesis involving YAP/β-catenin signaling | ||
Bone Osteoblast-derived exosomes promote osteogenic differentiation of osteosarcoma cells via URG4/Wnt signaling pathway | ||
Cells DPPA2/4 Promote the Pluripotency and Proliferation of Bovine Extended Pluripotent Stem Cells by Upregulating the PI3K/AKT/GSK3β/β-Catenin Signaling Pathway | ||
Exp Mol Med Advanced glycation end products promote meniscal calcification by activating the mTOR-ATF4 positive feedback loop | ||
Am J Chin Med Astragaloside IV Ameliorates Colonic Adenomatous Polyps Development by Orchestrating Gut Bifidobacterium and Serum Metabolome |
