Phospho-Chk1 (Ser296) Polyclonal antibody

Phospho-Chk1 (Ser296) Polyclonal Antibody for WB, ELISA
Cat No. 28805-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

CHEK1, CHK1, Checkpoint kinase-1, CHEK 1, Chk 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inCalyculin A treated HEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy CHK1 Ready-To-Use IHC Kit. CHK1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 5 publications below

产品信息

28805-1-AP targets Phospho-Chk1 (Ser296) in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CHK1 checkpoint homolog (S. pombe)
别名 CHEK1, CHK1, Checkpoint kinase-1, CHEK 1, Chk 1
计算分子量 54 kDa
观测分子量55 kDa
GenBank蛋白编号BC004202
基因名称 Chk1
Gene ID (NCBI) 1111
RRIDAB_2881215
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14757
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

In response to DNA damage, mammalian cells prevent cell cycle progression through the control of critical cell cycle regulators. CHK1 (synonym: CHEK1), a homolog of the Schizosaccharomyces pombe Chk1 protein kinase, is required for the DNA damage checkpoint. Human Chk1 protein is modified in response to DNA damage. In vitro Chk1 binds to and phosphorylate the dual-specificity protein phosphatases Cdc25A, Cdc25B, and Cdc25C, which control cell cycle transitions by dephosphorylating cyclin-dependent kinases. CHK1 can be autophosphorylated (PMID:22941630) and ubiquitinated (PMID:19276361). Activation of Chk1 involves phosphorylation at Ser317 and Ser345 by ATM/ATR, followed by autophosphorylation of Ser296. Activation occurs in response to blocked DNA replication and certain forms of genotoxic stress.

实验方案

Product Specific Protocols
WB protocol for Phospho-Chk1 (Ser296) antibody 28805-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Arch Toxicol

SIRT3-dependent mitochondrial redox homeostasis mitigates CHK1 inhibition combined with gemcitabine treatment induced cardiotoxicity in hiPSC-CMs and mice

Authors - Jia-Wen Chen
humanWB

Cell Prolif

eIF3a-PPP2R5A-mediated ATM/ATR dephosphorylation is essential for irinotecan-induced DNA damage response.

Authors - Chao Mei
humanWB

Adv Sci (Weinh)

Transferrin Receptor-Mediated Iron Uptake Promotes Colon Tumorigenesis

Authors - Hyeoncheol Kim
humanWB

Research (Wash D C)

Nanoengineered Gallium Ion Incorporated Formulation for Safe and Efficient Reversal of PARP Inhibition and Platinum Resistance in Ovarian Cancer

Authors - Yangyang Li
mouseWB

Cell Death Discov

Tert promotes cardiac regenerative repair after MI through alleviating ROS-induced DNA damage response in cardiomyocyte

Authors - Xiaomin Wei
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