Phospho-Beta Catenin (Ser33) Recombinant antibody

Phospho-Beta Catenin (Ser33) Uni-rAbTM Recombinant Antibody for WB, ELISA, FC (Intra)
Cat No. 80067-1-RR

产品说明书

CloneNo. 3K1

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse, Rat and More (2)

应用

WB, ELISA, FC (Intra)

Beta catenin, Catenin beta 1, beta-Catenin, B-catenin, b cat

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inPC-3 cells, HT-29 cells, Calyculin A treated HT-29 cells, Calyculin A treated PC-3 cells
Positive FC (Intra) detected inCalyculin A treated PC-3 cells, PC-3 cells
Planning an IHC experiment? We recommend our IHCeasy Beta Catenin Ready-To-Use IHC Kit. Beta Catenin primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 9 publications below

产品信息

80067-1-RR targets Phospho-Beta Catenin (Ser33) in WB, FC (Intra), ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, ELISA, FC (Intra) Application Description
文献引用应用WB
经测试反应性 Human, Mouse, Rat
文献引用反应性human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 catenin (cadherin-associated protein), beta 1, 88kDa
别名 Beta catenin, Catenin beta 1, beta-Catenin, B-catenin, b cat
计算分子量 781 aa, 86 kDa
观测分子量90 kDa
GenBank蛋白编号BC058926
基因名称 Beta Catenin
Gene ID (NCBI) 1499
ENSEMBL Gene IDENSG00000168036
RRIDAB_2918861
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP35222
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222). CK1 phosphorylates β-Catenin at Ser45. This phosphorylation event primes β-Catenin for subsequent phosphorylation by GSK-3β. GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41. Mutations at these sites result in the stabilization of β-Catenin protein levels and have been found in many tumor cell lines .

实验方案

Product Specific Protocols
WB protocol for Phospho-Beta Catenin (Ser33) antibody 80067-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Ethnopharmacol

Mechanism of Buyang Huanwu Decoction mediating Cav1-regulated Wnt pathway to promote neural regeneration in cerebral ischemic mice

Authors - Yin OuYang
mouseWB

Mediators Inflamm

Liraglutide Attenuates Hepatic Oxidative Stress, Inflammation, and Apoptosis in Streptozotocin-Induced Diabetic Mice by Modulating the Wnt/β-Catenin Signaling Pathway

Authors - Jie Yu
humanWB

Front Pharmacol

Autophagy Blockade by Ai Du Qing Formula Promotes Chemosensitivity of Breast Cancer Stem Cells Via GRP78/β-Catenin/ABCG2 Axis.

Authors - Mianmian Liao
humanWB

Acta Biochim Biophys Sin (Shanghai)

Targeting GRP78 enhances the sensitivity of HOS osteosarcoma cells to pyropheophorbide-α methyl ester-mediated photodynamic therapy via the Wnt/β-catenin signaling pathway.

Authors - Qiang Zuo
mouseWB

Exp Ther Med

Insulin and liraglutide attenuate brain pathology in diabetic mice by enhancing the Wnt/β-catenin signaling pathway.

Authors - Yuan Zhao
humanWB

Biochem Pharmacol

SMURF1 leads to the β-catenin signaling-mediated progression of esophageal squamous carcinoma by losing PATZ1-induced CCNG2 transcription

Authors - Lingling Chen
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