Phospho-Beta Catenin (Ser33) Recombinant antibody

Phospho-Beta Catenin (Ser33) Uni-rAbTM Recombinant Antibody for WB, ELISA, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, ELISA, FC (Intra)

Conjugate

Unconjugated

CloneNo.

3K1

Cat no : 80067-1-RR

Print datasheet

Synonyms

Beta catenin, Catenin beta 1, beta-Catenin, B-catenin, b cat



经过测试的应用

Positive WB detected inPC-3 cells, HT-29 cells, Calyculin A treated HT-29 cells, Calyculin A treated PC-3 cells
Positive FC (Intra) detected inCalyculin A treated PC-3 cells, PC-3 cells
Planning an IHC experiment? We recommend our IHCeasy Beta Catenin Ready-To-Use IHC Kit. Beta Catenin primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 8 publications below

产品信息

80067-1-RR targets Phospho-Beta Catenin (Ser33) in WB, FC (Intra), ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, ELISA, FC (Intra) Application Description
Cited ApplicationsWB
Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name catenin (cadherin-associated protein), beta 1, 88kDa
Synonyms Beta catenin, Catenin beta 1, beta-Catenin, B-catenin, b cat
Calculated Molecular Weight 781 aa, 86 kDa
Observed Molecular Weight90 kDa
GenBank Accession NumberBC058926
Gene Symbol Beta Catenin
Gene ID (NCBI) 1499
ENSEMBL Gene IDENSG00000168036
RRIDAB_2918861
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35222
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222). CK1 phosphorylates β-Catenin at Ser45. This phosphorylation event primes β-Catenin for subsequent phosphorylation by GSK-3β. GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41. Mutations at these sites result in the stabilization of β-Catenin protein levels and have been found in many tumor cell lines .

实验方案

Product Specific Protocols
WB protocol for Phospho-Beta Catenin (Ser33) antibody 80067-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Ethnopharmacol

Mechanism of Buyang Huanwu Decoction mediating Cav1-regulated Wnt pathway to promote neural regeneration in cerebral ischemic mice

Authors - Yin OuYang
mouseWB

Mediators Inflamm

Liraglutide Attenuates Hepatic Oxidative Stress, Inflammation, and Apoptosis in Streptozotocin-Induced Diabetic Mice by Modulating the Wnt/β-Catenin Signaling Pathway

Authors - Jie Yu
humanWB

Front Pharmacol

Autophagy Blockade by Ai Du Qing Formula Promotes Chemosensitivity of Breast Cancer Stem Cells Via GRP78/β-Catenin/ABCG2 Axis.

Authors - Mianmian Liao
humanWB

Acta Biochim Biophys Sin (Shanghai)

Targeting GRP78 enhances the sensitivity of HOS osteosarcoma cells to pyropheophorbide-α methyl ester-mediated photodynamic therapy via the Wnt/β-catenin signaling pathway.

Authors - Qiang Zuo
mouseWB

Exp Ther Med

Insulin and liraglutide attenuate brain pathology in diabetic mice by enhancing the Wnt/β-catenin signaling pathway.

Authors - Yuan Zhao
mouseWB

Environ Toxicol

Microplastics cause hepatotoxicity in diabetic mice by disrupting glucolipid metabolism via PP2A/AMPK/HNF4A and promoting fibrosis via the Wnt/β-catenin pathway

Authors - Xinxin Li