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Phospho-Cardiac Troponin I (Ser23/24) Polyclonal antibody

Phospho-Cardiac Troponin I (Ser23/24) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 30937-1-AP

Print datasheet

Synonyms

Cardiac troponin I, CMD2A, CMH7, cTnI, Phospho-Cardiac Troponin I (Ser23/24), RCM1, TNNC1, TNNI3, troponin I type 3 (cardiac), Troponin I, cardiac muscle

验证数据展示

  • WB analysis of mouse heart using 30937-1-AP

    WB analysis of mouse heart using 30937-1-AP

    Phosphatase inhibitor treated mouse heart tissue, λ phosphatase treated mouse heart tissue, and non-treated mouse heart tissue were subjected to SDS PAGE followed by western blot with 30937-1-AP (Phospho-TNNI3 (Ser23/24) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

经过测试的应用

Positive WB detected inmouse heart tissue, λ phosphatase treated mouse heart tissue
Planning an IHC experiment? We recommend our IHCeasy TNNI3 Ready-To-Use IHC Kit. TNNI3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30937-1-AP targets Phospho-Cardiac Troponin I (Ser23/24) in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name troponin I type 3 (cardiac)
Synonyms Cardiac troponin I, CMD2A, CMH7, cTnI, Phospho-Cardiac Troponin I (Ser23/24), RCM1, TNNC1, TNNI3, troponin I type 3 (cardiac), Troponin I, cardiac muscle
Calculated Molecular Weight 210 aa, 24 kDa
Observed Molecular Weight26 kDa
GenBank Accession NumberBC096165
Gene Symbol TNNI3
Gene ID (NCBI) 7137
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP19429
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cardiac Troponin I (cTnI) is produced by cardiac muscle. To date, most investigative effort in phosphorylation mediated regulation of cTnI function has focused on the actions of PKA and PKC. Evidence from studies in a variety of systems, ranging from reconstituted myofilament proteins to cultured myocytes or transgenic animals with heterologous expression of modified cTnI proteins, indicates that PKA-mediated functional effects (eg, decreased myofilament Ca2 sensitivity) are mediated principally through the phosphorylation of Ser22/23 (equivalent to Ser23/24, including the initiating Met, in the rodent sequence) within the unique N-terminal extension of cTnI. (PMID: 15514163)

实验方案

Product Specific Protocols
WB protocol for Phospho-Cardiac Troponin I (Ser23/24) antibody 30937-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols