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Phospho-Chk1 (Ser317) Polyclonal antibody, PBS Only

Phospho-Chk1 (Ser317) Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28807-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 28807-1-AP (same clone as 28807-1-PBS)

    WB analysis using 28807-1-AP (same clone as 28807-1-PBS)

    Non-treated HEK-293T and UV treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 28807-1-AP (Phospho-Chk1 (Ser317) antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 28807-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CHK1 Ready-To-Use IHC Kit. CHK1 primary antibody included.

产品信息

28807-1-PBS targets Phospho-Chk1 (Ser317) in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CHK1 checkpoint homolog (S. pombe)
Synonyms
Calculated Molecular Weight 54 kDa
Observed Molecular Weight55 kDa
GenBank Accession NumberBC004202
Gene Symbol Chk1
Gene ID (NCBI) 1111
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14757
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The checkpoint kinase 1 (Chk1) is a conserved kinase that imposes cell cycle delays in response to impediments to DNA replication. Chk1 kinase is downstream of the ATR kinase. ATR phosphorylates Chk1 kinase and other proteins to ensure replication is being blocked as to avoid replication fork collapse and DNA damage. Activation of Chk1 involves phosphorylation at Ser317 and Ser345 by ATM/ATR, followed by autophosphorylation of Ser296. In vitro Chk1 binds to and phosphorylate the dual-specificity protein phosphatases Cdc25A, Cdc25B, and Cdc25C, which control cell cycle transitions by dephosphorylating cyclin-dependent kinases. (PMID:22941630, PMID: 32571801, PMID:19276361)