Phospho-Chk1 (Ser345) Polyclonal antibody

Phospho-Chk1 (Ser345) Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 28803-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

CHEK1, CHK1, CHK1 checkpoint homolog, Chk 1, CHEK 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inCalyculin A treated NIH/3T3 cells, Calyculin A treated HeLa cells
Positive IF/ICC detected inCalyculin A treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy CHK1 Ready-To-Use IHC Kit. CHK1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 4 publications below

产品信息

28803-1-AP targets Phospho-Chk1 (Ser345) in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CHK1 checkpoint homolog (S. pombe)
别名 CHEK1, CHK1, CHK1 checkpoint homolog, Chk 1, CHEK 1
计算分子量 54 kDa
观测分子量50 kDa
GenBank蛋白编号BC004202
基因名称 Chk1
Gene ID (NCBI) 1111
RRIDAB_2918206
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14757
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The checkpoint kinase 1 (Chk1) is a conserved kinase that imposes cell cycle delays in response to impediments to DNA replication. Chk1 kinase is downstream of the ATR kinase. ATR phosphorylates Chk1 kinase and other proteins to ensure replication is being blocked as to avoid replication fork collapse and DNA damage. Activation of Chk1 involves phosphorylation at Ser317 and Ser345 by ATM/ATR, followed by autophosphorylation of Ser296. In vitro Chk1 binds to and phosphorylate the dual-specificity protein phosphatases Cdc25A, Cdc25B, and Cdc25C, which control cell cycle transitions by dephosphorylating cyclin-dependent kinases. (PMID:22941630, PMID: 32571801, PMID:19276361)

实验方案

Product Specific Protocols
WB protocol for Phospho-Chk1 (Ser345) antibody 28803-1-APDownload protocol
IF protocol for Phospho-Chk1 (Ser345) antibody 28803-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Transl Oncol

The ATR inhibitor VE-821 increases the sensitivity of gastric cancer cells to cisplatin

Authors - Haochen Su
humanWB

Phytomedicine

Epiberberine induced p53/p21-dependent G2/M cell cycle arrest and cell apoptosis in gastric cancer cells by activating γ-aminobutyric acid receptor- β3

Authors - Mengmeng Li
humanWB

J Exp Clin Cancer Res

HIF-1α-HPRT1 axis promotes tumorigenesis and gefitinib resistance by enhancing purine metabolism in EGFR-mutant lung adenocarcinoma

Authors - Pengyu Geng
humanWB

Cancer Sci

P2RY6 deletion promotes UVB-induced skin carcinogenesis by activating the PI3K/AKT signal pathway

Authors - Peng Xu
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