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验证数据展示

  • WB analysis using 29715-1-AP

    WB analysis using 29715-1-AP

    Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 29715-1-AP (Phospho-Cofilin (Ser3) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as a loading control.

  • IF Staining of HeLa using 29715-1-AP

    IF Staining of HeLa using 29715-1-AP

    Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated HeLa cells using Phospho-Cofilin (Ser3) antibody (29715-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Phospho-Cofilin (Ser3) Polyclonal antibody

Phospho-Cofilin (Ser3) Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 29715-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

18 kDa phosphoprotein, CFL, CFL1, Cofilin 1, cofilin 1 (non muscle), Cofilin, non muscle isoform, p18, Phospho-Cofilin (Ser3)

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inλ phosphatase treated HeLa cells, HeLa cells
Positive IF/ICC detected inλ phosphatase treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy CFL1 Ready-To-Use IHC Kit. CFL1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29715-1-AP targets Phospho-Cofilin (Ser3) in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cofilin 1 (non-muscle)
Synonyms 18 kDa phosphoprotein, CFL, CFL1, Cofilin 1, cofilin 1 (non muscle), Cofilin, non muscle isoform, p18, Phospho-Cofilin (Ser3)
Calculated Molecular Weight 18 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC012318
Gene Symbol Cofilin
Gene ID (NCBI) 1072
RRIDAB_2935421
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP23528
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of the ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation. Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm.

实验方案

Product Specific Protocols
WB protocol for Phospho-Cofilin (Ser3) antibody 29715-1-APDownload protocol
IF protocol for Phospho-Cofilin (Ser3) antibody 29715-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols