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验证数据展示

  • WB analysis using 30278-1-AP

    WB analysis using 30278-1-AP

    Non-treated HeLa and EGF treated HeLa cells were subjected to SDS PAGE followed by western blot with 30278-1-AP (Phospho-EGFR (Tyr1092) antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin antibody as loading control.

Phospho-EGFR (Tyr1092) Polyclonal antibody

Phospho-EGFR (Tyr1092) Polyclonal Antibody for WB, ELISA
Cat No. 30278-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

EGFR, ERBB, ERBB1, HER1, mENA, Phospho-EGFR (Tyr1068), Phospho-EGFR (Tyr1092), PIG61, Proto oncogene c ErbB 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inEGF treated HeLa cells

This antibody, also known as EGFR Tyr1068, is the site where the signal peptide is removed.

Planning an IHC experiment? We recommend our IHCeasy EGFR Ready-To-Use IHC Kit. EGFR primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30278-1-AP targets Phospho-EGFR (Tyr1092) in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
别名 EGFR, ERBB, ERBB1, HER1, mENA, Phospho-EGFR (Tyr1068), Phospho-EGFR (Tyr1092), PIG61, Proto oncogene c ErbB 1
计算分子量 1210 aa, 134 kDa
观测分子量170 kDa
GenBank蛋白编号BC094761
基因名称 EGFR
Gene ID (NCBI) 1956
RRIDAB_3085350
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP00533
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Epidermal Growth Factor Receptor (EGFR) is one of the receptor tyrosine kinases (RTKs), members of the ErbB/HER family which consists of ErbB1 (EGFR or HER1), ErbB2 (HER2 or Neu), ErbB3 (HER3), and ErbB4 (HER4). Aberrant activation of EGFR is among the most common oncogenic driving events in human cancer, which is mediated largely through different classes of genomic alterations within the EGFR gene. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. (PMID: 35164092, PMID: 29464683, PMID: 37085908). Some scientists call EGFR Tyr1092 as EGFR Tyr1068, the site where the signaling peptide is removed.

实验方案

Product Specific Protocols
WB protocol for Phospho-EGFR (Tyr1092) antibody 30278-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols