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验证数据展示

  • WB analysis using 30278-1-AP

    WB analysis using 30278-1-AP

    Non-treated HeLa and EGF treated HeLa cells were subjected to SDS PAGE followed by western blot with 30278-1-AP (Phospho-EGFR (Tyr1092) antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin antibody as loading control.

Phospho-EGFR (Tyr1092) Polyclonal antibody

Phospho-EGFR (Tyr1092) Polyclonal Antibody for WB, ELISA
Cat No. 30278-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

EGFR, ERBB, ERBB1, HER1, mENA, Phospho-EGFR (Tyr1068), Phospho-EGFR (Tyr1092), PIG61, Proto oncogene c ErbB 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inEGF treated HeLa cells

This antibody, also known as EGFR Tyr1068, is the site where the signal peptide is removed.

Planning an IHC experiment? We recommend our IHCeasy EGFR Ready-To-Use IHC Kit. EGFR primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30278-1-AP targets Phospho-EGFR (Tyr1092) in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Synonyms EGFR, ERBB, ERBB1, HER1, mENA, Phospho-EGFR (Tyr1068), Phospho-EGFR (Tyr1092), PIG61, Proto oncogene c ErbB 1
Calculated Molecular Weight 1210 aa, 134 kDa
Observed Molecular Weight170 kDa
GenBank Accession NumberBC094761
Gene Symbol EGFR
Gene ID (NCBI) 1956
RRIDAB_3085350
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP00533
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Epidermal Growth Factor Receptor (EGFR) is one of the receptor tyrosine kinases (RTKs), members of the ErbB/HER family which consists of ErbB1 (EGFR or HER1), ErbB2 (HER2 or Neu), ErbB3 (HER3), and ErbB4 (HER4). Aberrant activation of EGFR is among the most common oncogenic driving events in human cancer, which is mediated largely through different classes of genomic alterations within the EGFR gene. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. (PMID: 35164092, PMID: 29464683, PMID: 37085908). Some scientists call EGFR Tyr1092 as EGFR Tyr1068, the site where the signaling peptide is removed.

实验方案

Product Specific Protocols
WB protocol for Phospho-EGFR (Tyr1092) antibody 30278-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols