Phospho-EIF2S1 (Ser51) Monoclonal antibody
Phospho-EIF2S1 (Ser51) Monoclonal Antibody for FC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Rat, Mouse and More (1)
Applications
FC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
1A4A11
验证数据展示
经过测试的应用
Positive WB detected in | PC-3 cells, HeLa cells, Calyculin A treated HEK-293 cells, NIH/3T3 cells, HSC-T6 cells, Calyculin A treated HeLa cells, Calyculin A treated PC-3 cells, Calyculin A treated NIH/3T3 cells, Calyculin A treated HSC-T6 cells |
Positive FC detected in | Calyculin A treated PC-3 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Flow Cytometry (FC) | FC : 0.50 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 4 publications below |
产品信息
68023-1-Ig targets Phospho-EIF2S1 (Ser51) in WB, FC, ELISA applications and shows reactivity with Human, Rat, Mouse samples.
Tested Applications | FC, WB, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | Human, Rat, Mouse |
Cited Reactivity | human |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa |
Synonyms | EIF 2, eIF 2 alpha, EIF 2A, EIF 2alpha, EIF2, EIF2A, EIF2S1 (Ser51), EIF2S1 S51, EIF2S1 Ser51, Phospho-EIF2S1 (Ser51) |
Calculated Molecular Weight | 36 kDa |
Observed Molecular Weight | 36 kDa |
GenBank Accession Number | NM_004094 |
Gene Symbol | EIF2S1 |
Gene ID (NCBI) | 1965 |
RRID | AB_2918767 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P05198 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Phospho-EIF2S1 (Ser51) antibody 68023-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
bioRxiv Mitosis exit followed by death in interphase prevents the development of polyploid giant cancer cells | ||
Cell Death Dis AURKA emerges as a vulnerable target for KEAP1-deficient non-small cell lung cancer by activation of asparagine synthesis | ||
J Transl Med The microtubule targeting agent ST-401 triggers cell death in interphase and prevents the formation of polyploid giant cancer cells | ||
Environ Pollut Copper exposure induces mitochondrial dysfunction and hepatotoxicity via the induction of oxidative stress and PERK/ATF4 -mediated endoplasmic reticulum stress |