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  • Featured Product
  • KD/KO Validated

Phospho-EIF4B (Ser406) Recombinant antibody, PBS Only

Phospho-EIF4B (Ser406) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5G14

Cat no : 80059-1-PBS

Print datasheet

Synonyms

EIF4B, PRO1843, eIF-4B, EIF 4B, 5G14

验证数据展示

  • WB analysis using 80059-1-RR (same clone as 80059-1-PBS)

    WB analysis using 80059-1-RR (same clone as 80059-1-PBS)

    Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 80059-1-RR (Phospho-EIF4B (Ser406) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80059-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 80059-1-RR (same clone as 80059-1-PBS)

    WB analysis of HeLa using 80059-1-RR (same clone as 80059-1-PBS)

    WB result of Phospho-EIF4B (Ser406) antibody (80059-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Phospho-EIF4B (Ser406) transfected HeLa cells. This data was developed using the same antibody clone with 80059-1-PBS in a different storage buffer formulation.

产品信息

80059-1-PBS targets Phospho-EIF4B (Ser406) in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name eukaryotic translation initiation factor 4B
Synonyms EIF4B, PRO1843, eIF-4B, EIF 4B, 5G14
Calculated Molecular Weight 611 aa, 69 kDa
Observed Molecular Weight70-80 kDa
GenBank Accession NumberBC073154
Gene Symbol EIF4B
Gene ID (NCBI) 1975
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP23588
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EIF4B is one of the mammalian eukaryotic initiation factors (eIF) that are required for the ATP-dependent binding of mRNA to the 40 S ribosomal subunit, and the other eIF proteins are EIF4A, EIF4F. eIF4B is involved in translation of numerous proliferative or anti-apoptotic mRNAs with highly structured 5'UTR and subsequently affect cell growth and survival. It was reported that false expression and phosphorylation levels of eIF4B are involved in several tumors including breast cancer, cell lymphoblastic leukemia and diffuse large B-cell lymphoma (PMID: 26848623).