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验证数据展示

  • WB analysis using 80059-1-RR (same clone as 80059-1-PBS)

    WB analysis using 80059-1-RR (same clone as 80059-1-PBS)

    Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 80059-1-RR (Phospho-EIF4B (Ser406) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80059-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 80059-1-RR (same clone as 80059-1-PBS)

    WB analysis of HeLa using 80059-1-RR (same clone as 80059-1-PBS)

    WB result of Phospho-EIF4B (Ser406) antibody (80059-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Phospho-EIF4B (Ser406) transfected HeLa cells. This data was developed using the same antibody clone with 80059-1-PBS in a different storage buffer formulation.

  • IHC staining of Jurkat using 80059-1-RR (same clone as 80059-1-PBS)

    IHC staining of Jurkat using 80059-1-RR (same clone as 80059-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 80059-1-RR (Phospho-EIF4B (Ser406) antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80059-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Phospho-EIF4B (Ser406) Recombinant antibody, PBS Only

Phospho-EIF4B (Ser406) Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 80059-1-PBS

产品说明书

CloneNo. 5G14

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

EIF4B, PRO1843, eIF-4B, EIF 4B, 5G14

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

80059-1-PBS targets Phospho-EIF4B (Ser406) in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 eukaryotic translation initiation factor 4B
别名 EIF4B, PRO1843, eIF-4B, EIF 4B, 5G14
计算分子量 611 aa, 69 kDa
观测分子量70-80 kDa
GenBank蛋白编号BC073154
基因名称 EIF4B
Gene ID (NCBI) 1975
RRIDAB_2918859
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP23588
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EIF4B is one of the mammalian eukaryotic initiation factors (eIF) that are required for the ATP-dependent binding of mRNA to the 40 S ribosomal subunit, and the other eIF proteins are EIF4A, EIF4F. eIF4B is involved in translation of numerous proliferative or anti-apoptotic mRNAs with highly structured 5'UTR and subsequently affect cell growth and survival. It was reported that false expression and phosphorylation levels of eIF4B are involved in several tumors including breast cancer, cell lymphoblastic leukemia and diffuse large B-cell lymphoma (PMID: 26848623).