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验证数据展示

  • WB analysis using 28781-1-AP

    WB analysis using 28781-1-AP

    Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 28781-1-AP (Phospho-EIF4B (Ser422) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Phospho-EIF4B (Ser422) Polyclonal antibody

Phospho-EIF4B (Ser422) Polyclonal Antibody for WB, ELISA
Cat No. 28781-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

EIF 4B, EIF4B, Phospho-EIF4B (Ser422), PRO1843

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCalyculin A treated HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28781-1-AP targets Phospho-EIF4B (Ser422) in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 4B
别名 EIF 4B, EIF4B, Phospho-EIF4B (Ser422), PRO1843
计算分子量 611 aa, 69 kDa
观测分子量70-80 kDa
GenBank蛋白编号BC073154
基因名称 EIF4B
Gene ID (NCBI) 1975
RRIDAB_2935409
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP23588
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EIF4B is one of the mammalian eukaryotic initiation factors (eIF) that are required for the ATP-dependent binding of mRNA to the 40 S ribosomal subunit, and the other EIF proteins are EIF4A, EIF4F. EIF4B is involved in translation of numerous proliferative or anti-apoptotic mRNAs with highly structured 5'UTR and subsequently affect cell growth and survival. It was reported that false expression and phosphorylation levels of EIF4B are involved in several tumors including breast cancer, cell lymphoblastic leukemia and diffuse large B-cell lymphoma (PMID: 26848623). Ser422 is regarded as the major modification site of EIF4B that is phosphorylated in response to amino-acid refeeding(PMID: 22750809).

实验方案

Product Specific Protocols
WB protocol for Phospho-EIF4B (Ser422) antibody 28781-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols