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Phospho-EIF4B (Ser422) Recombinant antibody

Phospho-EIF4B (Ser422) Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1K3

Cat no : 82550-1-RR

Print datasheet

Synonyms

EIF 4B, EIF4B, Phospho-EIF4B (Ser422), PRO1843

验证数据展示

  • WB analysis using 82550-1-RR

    WB analysis using 82550-1-RR

    Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 82550-1-RR (Phospho-EIF4B (Ser422) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody as loading control.

经过测试的应用

Positive WB detected inCalyculin A treated HeLa cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82550-1-RR targets Phospho-EIF4B (Ser422) in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name eukaryotic translation initiation factor 4B
Synonyms EIF 4B, EIF4B, Phospho-EIF4B (Ser422), PRO1843
Calculated Molecular Weight 611 aa, 69 kDa
Observed Molecular Weight70-80 kDa
GenBank Accession NumberBC073154
Gene Symbol EIF4B
Gene ID (NCBI) 1975
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP23588
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EIF4B is one of the mammalian eukaryotic initiation factors (eIF) that are required for the ATP-dependent binding of mRNA to the 40 S ribosomal subunit, and the other EIF proteins are EIF4A, EIF4F. EIF4B is involved in translation of numerous proliferative or anti-apoptotic mRNAs with highly structured 5'UTR and subsequently affect cell growth and survival. It was reported that false expression and phosphorylation levels of EIF4B are involved in several tumors including breast cancer, cell lymphoblastic leukemia and diffuse large B-cell lymphoma (PMID: 26848623). Ser422 is regarded as the major modification site of EIF4B that is phosphorylated in response to amino-acid refeeding(PMID: 22750809).

实验方案

Product Specific Protocols
WB protocol for Phospho-EIF4B (Ser422) antibody 82550-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols