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Phospho-FOXO1 (Ser319) Polyclonal antibody

Phospho-FOXO1 (Ser319) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 28757-1-AP

Print datasheet

Synonyms

FKH1, FKHR, forkhead box O1, Forkhead box protein O1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma, FOXO1, FOXO1A, Phospho-FOXO1 (Ser319)

验证数据展示

  • WB analysis using 28757-1-AP

    WB analysis using 28757-1-AP

    Non-treated MCF-7 cells and Cobalt Chloride treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 28757-1-AP (Phospho-FOXO1 (Ser319) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin antibody as loading control.

经过测试的应用

Positive WB detected inMCF-7 cells, Cobalt Chloride treated MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy FOXO1 Ready-To-Use IHC Kit. FOXO1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28757-1-AP targets Phospho-FOXO1 (Ser319) in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity Human
Cited Reactivityhuman, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name forkhead box O1
Synonyms FKH1, FKHR, forkhead box O1, Forkhead box protein O1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma, FOXO1, FOXO1A, Phospho-FOXO1 (Ser319)
Calculated Molecular Weight 655 aa, 70 kDa
Observed Molecular Weight 70-80 kDa
GenBank Accession NumberBC021981
Gene Symbol FOXO1
Gene ID (NCBI) 2308
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12778
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The forkhead box class O (FoxO) family in mammals is comprised of the evolutionally highly conserved forkhead transcription factors, FoxO1, FoxO3a, FoxO4, and FoxO6. FoxO1 is highly expressed in lymphoid cells, especially in T and B cells. FoxO1 phosphorylation determines the transcriptional activity and cellular localization of FoxO1. Results showed that GLK-IKKβ signaling triggered the nuclear export of FoxO1. (PMID: 35966593)

实验方案

Product Specific Protocols
WB protocol for Phospho-FOXO1 (Ser319) antibody 28757-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

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