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Phospho-FRS2 (Tyr436) Polyclonal antibody

Phospho-FRS2 (Tyr436) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31352-1-AP

Print datasheet

Synonyms

FGFR signaling adaptor SNT, FGFR substrate 2, FRS2, FRS2A, FRS2alpha, Phospho-FRS2 (Tyr436), SNT, SNT 1, SNT1

验证数据展示

  • WB analysis using 31352-1-AP

    WB analysis using 31352-1-AP

    Non-treated NIH/3T3 cells and bFGF treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 31352-1-AP (Phospho-FRS2 (Tyr436) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as loading control.

经过测试的应用

Positive WB detected inbFGF treated NIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31352-1-AP targets Phospho-FRS2 (Tyr436) in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fibroblast growth factor receptor substrate 2
Synonyms FGFR signaling adaptor SNT, FGFR substrate 2, FRS2, FRS2A, FRS2alpha, Phospho-FRS2 (Tyr436), SNT, SNT 1, SNT1
Calculated Molecular Weight 60 kDa
Observed Molecular Weight80-85 kDa
GenBank Accession NumberBC021562
Gene Symbol FRS2
Gene ID (NCBI) 10818
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WU20
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Fibroblast growth factor substrate 2 (FRS2), a lipid-anchored docking protein that is phosphorylated upon activation of FGFR, is critical for recruitment of downstream signaling molecules and links the FGFRs to the Ras/Mek/Erk pathway and the PI3-Kinase/Akt pathway. Studies have found that the phosphotyrosine binding domain of FRS-2 directly binds the Trk receptors at the same phosphotyrosine residue that binds the signaling adapter Shc, suggesting a model in which competitive binding between FRS-2 and Shc regulates differentiation versusproliferation. (PMID: 19053057, PMID: 10092678)

实验方案

Product Specific Protocols
WB protocol for Phospho-FRS2 (Tyr436) antibody 31352-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols