验证数据展示
经过测试的应用
| Positive WB detected in | bFGF treated NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31352-1-AP targets Phospho-FRS2 (Tyr436) in WB, ELISA applications and shows reactivity with Human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | fibroblast growth factor receptor substrate 2 |
| 别名 | FGFR signaling adaptor SNT, FGFR substrate 2, FRS2, FRS2A, FRS2alpha, Phospho-FRS2 (Tyr436), SNT, SNT 1, SNT1 |
| 计算分子量 | 60 kDa |
| 观测分子量 | 80-85 kDa |
| GenBank蛋白编号 | BC021562 |
| 基因名称 | FRS2 |
| Gene ID (NCBI) | 10818 |
| RRID | AB_3669956 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8WU20 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Fibroblast growth factor substrate 2 (FRS2), a lipid-anchored docking protein that is phosphorylated upon activation of FGFR, is critical for recruitment of downstream signaling molecules and links the FGFRs to the Ras/Mek/Erk pathway and the PI3-Kinase/Akt pathway. Studies have found that the phosphotyrosine binding domain of FRS-2 directly binds the Trk receptors at the same phosphotyrosine residue that binds the signaling adapter Shc, suggesting a model in which competitive binding between FRS-2 and Shc regulates differentiation versusproliferation. (PMID: 19053057, PMID: 10092678)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-FRS2 (Tyr436) antibody 31352-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |