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验证数据展示

  • WB analysis using 28875-1-AP

    WB analysis using 28875-1-AP

    Non-treated PC-3 and Calyculin A treated treated PC-3 cells were subjected to SDS PAGE followed by western blot with 28875-1-AP (Phospho-GSK3A (Ser21) antibody) at dilution of 1:3000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Phospho-GSK3A (Ser21) Polyclonal antibody

Phospho-GSK3A (Ser21) Polyclonal Antibody for WB, ELISA
Cat No. 28875-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

DKFZp686D0638, GSK 3 alpha, GSK3A, Phospho-GSK3A (Ser21)

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCalyculin A treated PC-3 cells
Planning an IHC experiment? We recommend our IHCeasy GSK3A Ready-To-Use IHC Kit. GSK3A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28875-1-AP targets Phospho-GSK3A (Ser21) in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glycogen synthase kinase 3 alpha
别名 DKFZp686D0638, GSK 3 alpha, GSK3A, Phospho-GSK3A (Ser21)
计算分子量 483 aa, 51 kDa
观测分子量51 kDa
GenBank蛋白编号BC027984
基因名称 GSK3A
Gene ID (NCBI) 2931
RRIDAB_2918211
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP49840
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GSK3A (glycogen synthase kinase 3 alpha), one of the two functional isoforms encoded by the GSK3 gene, is a serine/threonine protein kinase that is involved in cell-cycle progression, differentiation, and apoptosis (PMID: 27049759). Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin.GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3A and Ser9 of GSK-3B. GSK3A acts as a tumor suppressor and has important roles in acute myeloid leukemia (AML), prostate cancer, and pancreatic cancer (PMID: 22326953, 22539113, 23547054).

实验方案

Product Specific Protocols
WB protocol for Phospho-GSK3A (Ser21) antibody 28875-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols