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Phospho-GSK3A (Ser21) Polyclonal antibody

Phospho-GSK3A (Ser21) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28875-1-AP

Print datasheet

Synonyms

DKFZp686D0638, GSK 3 alpha, GSK3A, Phospho-GSK3A (Ser21)

验证数据展示

  • WB analysis using 28875-1-AP

    WB analysis using 28875-1-AP

    Non-treated PC-3 and Calyculin A treated treated PC-3 cells were subjected to SDS PAGE followed by western blot with 28875-1-AP (Phospho-GSK3A (Ser21) antibody) at dilution of 1:3000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

经过测试的应用

Positive WB detected inCalyculin A treated PC-3 cells
Planning an IHC experiment? We recommend our IHCeasy GSK3A Ready-To-Use IHC Kit. GSK3A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28875-1-AP targets Phospho-GSK3A (Ser21) in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glycogen synthase kinase 3 alpha
Synonyms DKFZp686D0638, GSK 3 alpha, GSK3A, Phospho-GSK3A (Ser21)
Calculated Molecular Weight 483 aa, 51 kDa
Observed Molecular Weight51 kDa
GenBank Accession NumberBC027984
Gene Symbol GSK3A
Gene ID (NCBI) 2931
RRIDAB_2918211
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49840
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GSK3A (glycogen synthase kinase 3 alpha), one of the two functional isoforms encoded by the GSK3 gene, is a serine/threonine protein kinase that is involved in cell-cycle progression, differentiation, and apoptosis (PMID: 27049759). Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin.GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3A and Ser9 of GSK-3B. GSK3A acts as a tumor suppressor and has important roles in acute myeloid leukemia (AML), prostate cancer, and pancreatic cancer (PMID: 22326953, 22539113, 23547054).

实验方案

Product Specific Protocols
WB protocol for Phospho-GSK3A (Ser21) antibody 28875-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols