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Host
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Species Reactivity
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Species Reactivity
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Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 67558-1-Ig (same clone as 67558-1-PBS)

    WB analysis using 67558-1-Ig (same clone as 67558-1-PBS)

    Non-treated and Calyculin A treated cell lysates were subjected to SDS PAGE followed by western blot with 67558-1-Ig (Phospho-GSK3B (Ser9) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67558-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67558-1-Ig (same clone as 67558-1-PBS)

    IHC staining of human liver cancer using 67558-1-Ig (same clone as 67558-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67558-1-Ig (GSK3B-phospho-S9 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67558-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GSK3B antibody included
  • IF Staining of PC-3 using 67558-1-Ig (same clone as 67558-1-PBS)

    IF Staining of PC-3 using 67558-1-Ig (same clone as 67558-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Calyculin A treated PC-3 cells and non-treated PC-3 cells using Phospho-GSK3B (Ser9) antibody (67558-1-Ig, Clone: 1C9E2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67558-1-PBS in a different storage buffer formulation.

  • WB analysis using 67558-1-Ig (same clone as 67558-1-PBS)

    WB analysis using 67558-1-Ig (same clone as 67558-1-PBS)

    Non-treated and Calyculin A treated PC-3 were subjected to SDS PAGE followed by western blot with 67558-1-Ig (Phospho-GSK3B (Ser9) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH Monoclonal antibody (60004-1-Ig) as loading control. This data was developed using the same antibody clone with 67558-1-PBS in a different storage buffer formulation.

Phospho-GSK3B (Ser9) Monoclonal antibody, PBS Only

Phospho-GSK3B (Ser9) Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67558-1-PBS

产品说明书

CloneNo. 1C9E2

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IHC, IF/ICC, ELISA

别名

GSK 3 beta, GSK3A B, GSK3B, GSK3B 216P, GSK3B S9, GSK3B Ser9, gsk3beta, Phospho-GSK3B (Ser9), Ser9

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy GSK3B Ready-To-Use IHC Kit. GSK3B primary antibody included.

产品信息

67558-1-PBS targets Phospho-GSK3B (Ser9) in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 glycogen synthase kinase 3 beta
别名 GSK 3 beta, GSK3A B, GSK3B, GSK3B 216P, GSK3B S9, GSK3B Ser9, gsk3beta, Phospho-GSK3B (Ser9), Ser9
观测分子量 48 kDa
GenBank蛋白编号NM_002093
基因名称 GSK3B
Gene ID (NCBI) 2932
RRIDAB_2882772
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP49841
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .