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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 28855-1-AP (same clone as 28855-1-PBS)

    WB analysis using 28855-1-AP (same clone as 28855-1-PBS)

    Non-treated PC-3 cells, phosphatase inhibitor treated and λ phosphatase PC-3 cells were subjected to SDS PAGE followed by western blot with 28855-1-AP (Phospho-GYS1 (Ser641) antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 28855-1-PBS in a different storage buffer formulation.

  • IF Staining of PC-3 using 28855-1-AP (same clone as 28855-1-PBS)

    IF Staining of PC-3 using 28855-1-AP (same clone as 28855-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated PC-3 cells using Phospho-GYS1 (Ser641) antibody (28855-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28855-1-PBS in a different storage buffer formulation.

Phospho-GYS1 (Ser641) Polyclonal antibody, PBS Only

Phospho-GYS1 (Ser641) Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 28855-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy GYS1 Ready-To-Use IHC Kit. GYS1 primary antibody included.

产品信息

28855-1-PBS targets Phospho-GYS1 (Ser641) in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glycogen synthase 1 (muscle)
别名
计算分子量 84 kDa
观测分子量84 kDa
GenBank蛋白编号BC007688
基因名称 GYS1
Gene ID (NCBI) 2997
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP13807
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Glycogen synthase 1 (GYS1, GS) catalyzes the key step of glycogen synthesis and plays an important role in glycogen metabolism in liver and muscle. In kidney tissues, glycogen synthase 1 (GYS1) is the most important rate-limiting enzyme functioning in the last step of glycogen synthesis. Pathologically, its deficiency has been shown to cause muscle glycogen storage disease type 0 and death. Studies of tumors showed that GYS1 was rapidly induced under hypoxic conditions and positively correlated with glycogen accumulation in glioblastoma, breast, and colon cancer cell lines. GYS1 is phosphorylated at nine sites and insulin stimulates dephosphorylation of glycogen synthase. Insulin stimulates dephosphorylation of glycogen synthase via PKB-mediated phosphorylation of GSK3. Phosphorylation of GSK3 decreases kinase activity which will decrease phosphorylation of GS and increase glycogens synthase fractional activity. (PMID: 32802186, PMID: 30443599, PMID: 22232606)