验证数据展示
经过测试的应用
| Positive WB detected in | Calyculin A treated PC-3 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
80046-3-RR targets Phospho-HSP27 (Ser78) in WB, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | human |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | heat shock 27kDa protein 1 |
| Synonyms | HSP27, HSPB1, Hsp25, HMN2B, Estrogen-regulated 24 kDa protein |
| Calculated Molecular Weight | 19-23 kDa |
| Observed Molecular Weight | 25-30 kDa |
| GenBank Accession Number | BC012768 |
| Gene Symbol | HSPB1 |
| Gene ID (NCBI) | 3315 |
| RRID | AB_3670462 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P04792 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HSPB1, also named as HSP27, belongs to the mammalian small HSP family widely expressed in cells including the cytoplasm and nucleus. It has been reported that HSPB1 plays a key role in stress resistance, actin organization and numerous biological processes. HSPB1 can be phosphorylated in different stimulation such as cytokines, growth factors and peptide hormones on the site of Ser15, Ser78 and Ser82 by MAPKAP2 kinase. The phosphorylation of HSPB1 leads to a decrease in the size of HSPB1 oligomers. HSPB1 is associated with amyotrophic lateral sclerosis (ALS) (PMID: 8542580, 11960785, 10383393, 32231288)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-HSP27 (Ser78) antibody 80046-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |