Phospho-Histone H2A.X (Ser139) Polyclonal antibody
Phospho-Histone H2A.X (Ser139) Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | UV treated HEK-293 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Sample-dependent, check data in validation data gallery | |
产品信息
29380-1-AP targets Phospho-Histone H2A.X (Ser139) in WB, ELISA applications and shows reactivity with Human samples.
| Tested Applications | WB, ELISA |
| Tested Reactivity | Human |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | H2A histone family, member X |
| Synonyms | H2A histone family, member X, H2A.X, H2A/X, H2AFX, H2AX, Histone H2A.x, Phospho-Histone H2A.X (Ser140), γH2AX |
| Calculated molecular weight | 15 kDa |
| Observed molecular weight | 15 kDa |
| GenBank accession number | BC013416 |
| Gene symbol | H2AFX |
| Uniprot | P16104 |
| Gene ID (NCBI) | 3014 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The histone variant H2AX is a major component of the DNA damage response (DDR), especially functioning in amplifying DNA damage signals. In response to DNA double-strand breaks (DSBs), H2AX is instantaneously phosphorylated at Ser140 (a form called cH2AX) by the kinases ATM and ATR and is progressively dephosphorylated at Tyr143 by the Eya1 and Eya3 tyrosine phosphatases, resulting in a temporal switch from a postulated diphosphorylated (pSer140, pTyr143) to monophosphorylated (pSer140) H2AX state. Because phosphorylation of H2AX at Ser 140 (γ-H2AX) is abundant, fast, and correlates well with each DSB, it is the most sensitive marker that can be used to examine the DNA damage produced and the subsequent repair of the DNA lesion. The phosphorylation site of H2AX, Ser140, has also been described as Ser139 in other literature, and they recognize the same amino acid site. (PMID: 22908299, PMID: 17615256, PMID:22941631)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-Histone H2A.X (Ser139) antibody 29380-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |