Phospho-INSR (Tyr1150/1151)/IGF1R (Tyr1135/1136) Polyclonal antibody

Phospho-INSR (Tyr1150/1151)/IGF1R (Tyr1135/1136) Polyclonal Antibody for WB, ELISA
Cat No. 31133-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human and More (1)

应用

WB, ELISA

CD220, HHF5, INSR, insulin receptor, IR

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inIGF-1 treated HEK-293T cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

31133-1-AP targets Phospho-INSR (Tyr1150/1151)/IGF1R (Tyr1135/1136) in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB
经测试反应性 Human
文献引用反应性mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 INSR
别名 CD220, HHF5, INSR, insulin receptor, IR
观测分子量95 kDa
GenBank蛋白编号BC117172
基因名称 INSR
Gene ID (NCBI) 3643
RRIDAB_3669868
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP06213
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Insulin plays a crucial role in brain functions such as memory improvement and energy metabolism. The INSR shares a high structural homology with the IGF1R (84% similarity in the tyrosine kinase domain, 45-65% in the ligand-binding domain, and more than 50% in the overall amino acid sequence). In addition, ligand-dependent activation of the INSR and IGF1R activates almost identical downstream signaling cascades. This antibody recognizes the phosphorylation of tyrosine 1150 and 1151 of INSR, as well as the phosphorylation of tyrosine 1135 and 1136 of IGF1R. ((PMID:24434591))

实验方案

Product Specific Protocols
WB protocol for Phospho-INSR (Tyr1150/1151)/IGF1R (Tyr1135/1136) antibody 31133-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Commun

Intestinal Nogo-B reduces GLP1 levels by binding to proglucagon on the endoplasmic reticulum to inhibit PCSK1 cleavage

Authors - Ke Gong
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