Phospho-IRF3 (Ser396) Polyclonal antibody

Phospho-IRF3 (Ser396) Polyclonal Antibody for WB, ELISA
Cat No. 29528-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (4)

应用

WB, ELISA and More (1)

IRF-3 (Ser396), IRF3 (Ser396), Phospho IRF3 (Ser396), Phospho-IRF3, Phospho-IRF-3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inLPS treated THP-1 cells
Planning an IHC experiment? We recommend our IHCeasy IRF3 Ready-To-Use IHC Kit. IRF3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29528-1-AP targets Phospho-IRF3 (Ser396) in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human, mouse, rat, pig, monkey
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 interferon regulatory factor 3
别名 IRF-3 (Ser396), IRF3 (Ser396), Phospho IRF3 (Ser396), Phospho-IRF3, Phospho-IRF-3
计算分子量 47 kDa
观测分子量50-60 kDa
GenBank蛋白编号BC009395
基因名称 IRF3
Gene ID (NCBI) 3661
RRIDAB_2935415
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14653
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interferons (IFNs) are a large family of multifunctional secreted proteins involved in antiviral defense, cell growth regulation, and immune activation(PMID: 18440553). A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. IRF-3 is activated through phosphorylation by the kinases IKKɛ and/or TBK1. Phosphorylation at Ser396 is essential for IRF-3 activation. After cytoplasmic IRF-3 is phosphorylated at Ser386 and Ser396, phosphorylated IRF-3 translocates into the nucleus. This phosphorylated IRF-3 activates IFN and IFN-responsive genes.

实验方案

Product Specific Protocols
WB protocol for Phospho-IRF3 (Ser396) antibody 29528-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigWB

Int J Biol Macromol

Role of OGDH in Atophagy-IRF3-IFN-β pathway during classical swine fever virus infection

Authors - Sen Zeng
humanWB

Int J Mol Sci

FHL2 Inhibits SARS-CoV-2 Replication by Enhancing IFN-β Expression through Regulating IRF-3

Authors - Zhiqiang Xu
humanWB

J Transl Med

Therapeutic efficacy of a novel self-assembled immunostimulatory siRNA combining apoptosis promotion with RIG-I activation in gliomas

Authors - Junxiao Chen
mouseWB

Adv Sci (Weinh)

T Cell-Derived Apoptotic Extracellular Vesicles Hydrolyze cGAMP to Alleviate Radiation Enteritis via Surface Enzyme ENPP1

Authors - Yang Zhou
humanWB

Vet Microbiol

Hunnivirus structural protein VP2 inhibits beta interferon production by targeting the IRF3 essential modulator

Authors - Xinyue Zhu
ratWB

Phytomedicine

Troxerutin dampened hypothalamic neuroinflammation via microglial IL-22/IL-22R1/IRF3 activation in dihydrotestosterone-induced polycystic ovary syndrome rats

Authors - Zixuan Gao
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