Phospho-IRF3 (Ser396) Polyclonal antibody
Phospho-IRF3 (Ser396) Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human and More (4)
Applications
WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | LPS treated THP-1 cells |
Planning an IHC experiment? We recommend our IHCeasy IRF3 Ready-To-Use IHC Kit. IRF3 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 6 publications below |
产品信息
29528-1-AP targets Phospho-IRF3 (Ser396) in WB, ELISA applications and shows reactivity with Human samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse, monkey, pig |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | interferon regulatory factor 3 |
| Synonyms | interferon regulatory factor 3, IRF 3, IRF3, Phospho-IRF3 (Ser396) |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 50-60 kDa |
| GenBank Accession Number | BC009395 |
| Gene Symbol | IRF3 |
| Gene ID (NCBI) | 3661 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14653 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Interferons (IFNs) are a large family of multifunctional secreted proteins involved in antiviral defense, cell growth regulation, and immune activation(PMID: 18440553). A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. IRF-3 is activated through phosphorylation by the kinases IKKɛ and/or TBK1. Phosphorylation at Ser396 is essential for IRF-3 activation. After cytoplasmic IRF-3 is phosphorylated at Ser386 and Ser396, phosphorylated IRF-3 translocates into the nucleus. This phosphorylated IRF-3 activates IFN and IFN-responsive genes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-IRF3 (Ser396) antibody 29528-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Biol Macromol Role of OGDH in Atophagy-IRF3-IFN-β pathway during classical swine fever virus infection | ||
Int J Mol Sci FHL2 Inhibits SARS-CoV-2 Replication by Enhancing IFN-β Expression through Regulating IRF-3 | ||
J Transl Med Therapeutic efficacy of a novel self-assembled immunostimulatory siRNA combining apoptosis promotion with RIG-I activation in gliomas | ||
Signal Transduct Target Ther The cGAS-STING pathway-dependent sensing of mitochondrial DNA mediates ocular surface inflammation | ||
Viruses Naringenin Improves Innate Immune Suppression after PRRSV Infection by Reactivating the RIG-I-MAVS Signaling Pathway, Promoting the Production of IFN-I | ||
Inflammopharmacology Rapamycin can alleviate the submandibular gland pathology of Sjögren's syndrome by limiting the activation of cGAS-STING signaling pathway |