Phospho-IRF3 (Ser396) Polyclonal antibody

Phospho-IRF3 (Ser396) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 29528-1-AP

Print datasheet

Synonyms

IRF-3 (Ser396), IRF3 (Ser396), Phospho IRF3 (Ser396), Phospho-IRF3, Phospho-IRF-3



经过测试的应用

Positive WB detected inLPS treated THP-1 cells
Planning an IHC experiment? We recommend our IHCeasy IRF3 Ready-To-Use IHC Kit. IRF3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29528-1-AP targets Phospho-IRF3 (Ser396) in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman, mouse, rat, pig, monkey
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interferon regulatory factor 3
Synonyms IRF-3 (Ser396), IRF3 (Ser396), Phospho IRF3 (Ser396), Phospho-IRF3, Phospho-IRF-3
Calculated Molecular Weight 47 kDa
Observed Molecular Weight50-60 kDa
GenBank Accession NumberBC009395
Gene Symbol IRF3
Gene ID (NCBI) 3661
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14653
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interferons (IFNs) are a large family of multifunctional secreted proteins involved in antiviral defense, cell growth regulation, and immune activation(PMID: 18440553). A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. IRF-3 is activated through phosphorylation by the kinases IKKɛ and/or TBK1. Phosphorylation at Ser396 is essential for IRF-3 activation. After cytoplasmic IRF-3 is phosphorylated at Ser386 and Ser396, phosphorylated IRF-3 translocates into the nucleus. This phosphorylated IRF-3 activates IFN and IFN-responsive genes.

实验方案

Product Specific Protocols
WB protocol for Phospho-IRF3 (Ser396) antibody 29528-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigWB

Int J Biol Macromol

Role of OGDH in Atophagy-IRF3-IFN-β pathway during classical swine fever virus infection

Authors - Sen Zeng
humanWB

Int J Mol Sci

FHL2 Inhibits SARS-CoV-2 Replication by Enhancing IFN-β Expression through Regulating IRF-3

Authors - Zhiqiang Xu
humanWB

J Transl Med

Therapeutic efficacy of a novel self-assembled immunostimulatory siRNA combining apoptosis promotion with RIG-I activation in gliomas

Authors - Junxiao Chen
mouseWB

Adv Sci (Weinh)

T Cell-Derived Apoptotic Extracellular Vesicles Hydrolyze cGAMP to Alleviate Radiation Enteritis via Surface Enzyme ENPP1

Authors - Yang Zhou
humanWB

Vet Microbiol

Hunnivirus structural protein VP2 inhibits beta interferon production by targeting the IRF3 essential modulator

Authors - Xinyue Zhu
ratWB

Phytomedicine

Troxerutin dampened hypothalamic neuroinflammation via microglial IL-22/IL-22R1/IRF3 activation in dihydrotestosterone-induced polycystic ovary syndrome rats

Authors - Zixuan Gao