• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 60666-1-Ig (same clone as 60666-1-PBS)

    WB analysis using 60666-1-Ig (same clone as 60666-1-PBS)

    Non-treated and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60666-1-Ig (Phospho-JNK (Thr183/Tyr185) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (HRP-66095) antibody as a loading control. This data was developed using the same antibody clone with 60666-1-PBS in a different storage buffer formulation.

  • WB analysis using 60666-1-Ig (same clone as 60666-1-PBS)

    WB analysis using 60666-1-Ig (same clone as 60666-1-PBS)

    Non-treated and Anisomycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 60666-1-Ig (Phospho-JNK (Thr183/Tyr185) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (HRP-66095) antibody as a loading control. This data was developed using the same antibody clone with 60666-1-PBS in a different storage buffer formulation.

  • IHC staining of Jurkat using 60666-1-Ig (same clone as 60666-1-PBS)

    IHC staining of Jurkat using 60666-1-Ig (same clone as 60666-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 60666-1-Ig (Phospho-JNK (Thr183/Tyr185) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60666-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MAPK8/JNK antibody included

Phospho-JNK (Thr183/Tyr185) Monoclonal antibody, PBS Only

Phospho-JNK (Thr183/Tyr185) Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 60666-1-PBS

产品说明书

CloneNo. 2H8F12

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

JNK, MAPK8, JNK 2, JNK 1, EC:2.7.11.24

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy MAPK8/JNK Ready-To-Use IHC Kit. MAPK8/JNK primary antibody included.

产品信息

60666-1-PBS targets Phospho-JNK (Thr183/Tyr185) in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 mitogen-activated protein kinase 8
别名 JNK, MAPK8, JNK 2, JNK 1, EC:2.7.11.24
计算分子量 48 kDa
观测分子量42 kDa, 50 kDa
GenBank蛋白编号NM_138982
基因名称 JNK
Gene ID (NCBI) 5599
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP45983
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. The JNK gene generates 10 forms of JNK through alternative splicing, and the protein encoded by the JNK gene has or does not have a COOH terminal, resulting in 46 kDa and 54 kDa proteins. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. The antibody can detect endogenous levels of p46 and p54 SAPK/JNK when phosphorylated at Thr183 and Tyr185. It will also react with JNK singly phosphorylated at Thr183.