验证数据展示
产品信息
60666-1-PBS targets Phospho-JNK (Thr183/Tyr185) in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, Indirect ELISA Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | mitogen-activated protein kinase 8 |
| Synonyms | JNK, MAPK8, JNK 2, JNK 1, EC:2.7.11.24 |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 42 kDa, 50 kDa |
| GenBank Accession Number | NM_138982 |
| Gene Symbol | JNK |
| Gene ID (NCBI) | 5599 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P45983 |
| Storage Buffer | PBS Only |
| Storage Conditions | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. The JNK gene generates 10 forms of JNK through alternative splicing, and the protein encoded by the JNK gene has or does not have a COOH terminal, resulting in 46 kDa and 54 kDa proteins. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. The antibody can detect endogenous levels of p46 and p54 SAPK/JNK when phosphorylated at Thr183 and Tyr185. It will also react with JNK singly phosphorylated at Thr183.
