验证数据展示
经过测试的应用
| Positive WB detected in | Calyculin A treated HeLa cells |
The antibody also detects the phosphorylated at Thr1041 of the LATS2.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 7 publications below |
产品信息
28998-1-AP targets Phospho-LATS1 (Thr1079) in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human |
| 文献引用反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | LATS, large tumor suppressor, homolog 1 (Drosophila) |
| 别名 | h warts, LATS1, Phospho-LATS1 (Thr1079), WARTS, WARTS protein kinase, wts |
| 计算分子量 | 15 kDa, 127 kDa |
| 观测分子量 | 140-150 kDa |
| GenBank蛋白编号 | BC015665 |
| 基因名称 | LATS1 |
| Gene ID (NCBI) | 9113 |
| RRID | AB_2923586 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95835 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The large tumour suppressor 1 (LATS1) acting as a tumour suppressor is a serine/threonine kinase of the AGC kinase family and is found to be down-regulated in various types of human cancers. Recently, LATS1 has been identified as a central player of the emerging tumour suppressive Hippo signaling pathway. In this pathway, the MST1/2 kinase phosphorylates activates LATS1/2 which, together with its co-factor MOB1, subsequently phosphorylates and inhibits the YAP transcriptional factor by preventing them from translocating to the nucleus. The antibody also detects the phosphorylated at Thr1041 of the LATS2. (PMID: 32460168, PMID: 30219064,PMID: 31792377)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-LATS1 (Thr1079) antibody 28998-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Biol Sci S100 Calcium Binding Protein A16 Promotes Cell Proliferation by triggering LATS1 ubiquitin degradation mediated by CUL4A ligase to inhibit Hippo pathway in Glioma development | ||
Cell Mol Biol Lett KIAA1429-mediated m6A modification of CHST11 promotes progression of diffuse large B-cell lymphoma by regulating Hippo-YAP pathway | ||
J Transl Med Modulation of SRC by SNTB1 activates the Hippo-YAP pathway during colon adenocarcinoma metastasis | ||
Biochem Biophys Res Commun IGF2BP1 enhances the stability of SIK2 mRNA through m6A modification to promote non-small cell lung cancer progression | ||
Exp Eye Res Targeting mechanics-induced trabecular meshwork dysfunction through YAP-TGFβ Ameliorates high myopia-induced ocular hypertension | ||
Discov Oncol GALNT2 targeted by miR-139-5p promotes proliferation of clear cell renal cell carcinoma via inhibition of LATS2 activation |