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Phospho-MEK2 (Thr394) Polyclonal antibody

Phospho-MEK2 (Thr394) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (3)

Applications

WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 28955-1-AP

Print datasheet

Synonyms

ERK activator kinase 2, FLJ26075, MAP kinase kinase 2, MAP2K2, MAPK/ERK kinase 2, MAPKK 2, MAPKK2, MEK 2, MEK2, MKK2, Phospho-MAP2K2 (Thr394), PRKMK2

验证数据展示

  • WB analysis using 28955-1-AP

    WB analysis using 28955-1-AP

    Non-treated NIH/3T3, phosphatase inhibitor treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28955-1-AP (Phospho-MEK2 (Thr394) antibody) at dilution of 1:1000 incubated at 4℃ overnight.

经过测试的应用

Positive WB detected inNIH/3T3 cells, λ phosphatase treated NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy MAP2K2 Ready-To-Use IHC Kit. MAP2K2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28955-1-AP targets Phospho-MEK2 (Thr394) in WB, IF, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity Human, Mouse
Cited Reactivityhuman, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitogen-activated protein kinase kinase 2
Synonyms ERK activator kinase 2, FLJ26075, MAP kinase kinase 2, MAP2K2, MAPK/ERK kinase 2, MAPKK 2, MAPKK2, MEK 2, MEK2, MKK2, Phospho-MAP2K2 (Thr394), PRKMK2
Calculated Molecular Weight 44 kDa
Observed Molecular Weight44 kDa
GenBank Accession NumberBC018645
Gene Symbol MAP2K2
Gene ID (NCBI) 5605
RRIDAB_2881235
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP36507
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway.

实验方案

Product Specific Protocols
WB protocol for Phospho-MEK2 (Thr394) antibody 28955-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Nat Immunol

Defining blood-induced microglia functions in neurodegeneration through multiomic profiling

Authors - Andrew S Mendiola
View Article
ratWB

Pharm Biol

Myrtenol improves brain damage and promotes angiogenesis in rats with cerebral infarction by activating the ERK1/2 signalling pathway.

Authors - Shengming Huang
View Article
humanWB

Int J Biol Sci

Dual role of CD73 as a signaling molecule and adenosine-generating enzyme in colorectal cancer progression and immune evasion

Authors - Weidong Lian
View Article