验证数据展示
经过测试的应用
| Positive WB detected in | Calyculin A treated HEK-293T cells |
Planning an IHC experiment? We recommend our IHCeasy MAP3K7/TAK1 Ready-To-Use IHC Kit. MAP3K7/TAK1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
产品信息
28958-1-AP targets Phospho-TAK1 (Thr187) in WB, ELISA applications and shows reactivity with Human samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | mitogen-activated protein kinase kinase kinase 7 |
| Synonyms | MAP3K7, Phospho-MAP3K7/TAK1 (Thr187), TAK1, TGF beta activated kinase 1, TGF1a |
| Calculated Molecular Weight | 579 aa, 64 kDa |
| Observed Molecular Weight | 75-85 kDa |
| GenBank Accession Number | BC017715 |
| Gene Symbol | TAK1 |
| Gene ID (NCBI) | 6885 |
| RRID | AB_2918222 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43318 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAP3K7(Mitogen-activated protein kinase kinase kinase 7) is also named as TAK1 and belongs to the MAP kinase kinase kinase subfamily.It plays an important role in the cascades of cellular responses evoked by changes in the environment.It has been linked to interleukin-1 receptor and tumor necrosis factor receptor signaling (PMID: 16186825).It has 4 isoforms (53-55 kDa; 64-70 kDa and 75-80 kDa)produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-TAK1 (Thr187) antibody 28958-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Proteomics Global Phosphoproteomics Unveils Kinase-regulated Networks in Systemic Lupus Erythematosus | ||
Leukemia Thrombin receptor activating peptide-6 decreases acute graft-versus-host disease through activating GPR15 | ||
Cell Mol Immunol SMAD7 expression in CAR-T cells improves persistence and safety for solid tumors | ||
Cell Death Dis CRLF1 bridges AKT and mTORC2 through SIN1 to inhibit pyroptosis and enhance chemo-resistance in ovarian cancer |