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Phospho-MARCKS (Ser167/170) Polyclonal antibody

Phospho-MARCKS (Ser167/170) Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29145-1-AP

Print datasheet

Synonyms

80K L, 80K L protein, FLJ14368, FLJ90045, MACS, MARCKS, Phospho-MARCKS (Ser167/170), PKCSL, PRKCSL

验证数据展示

  • WB analysis of HeLa using 29145-1-AP

    WB analysis of HeLa using 29145-1-AP

    Non-treated HeLa cells, phosphatase inhibitor treated and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 29145-1-AP (Phospho-MARCKS (Ser167/170) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

  • IF Staining of HeLa using 29145-1-AP

    IF Staining of HeLa using 29145-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed λ phosphatase treated HeLa cells using Phospho-MARCKS (Ser167/170) antibody (29145-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inλ phosphatase treated HeLa cells
Positive IF/ICC detected inλ phosphatase treated HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29145-1-AP targets Phospho-MARCKS (Ser167/170) in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myristoylated alanine-rich protein kinase C substrate
Synonyms 80K L, 80K L protein, FLJ14368, FLJ90045, MACS, MARCKS, Phospho-MARCKS (Ser167/170), PKCSL, PRKCSL
Calculated Molecular Weight 32 kDa
Observed Molecular Weight80 kDa
GenBank Accession NumberBC089040
Gene Symbol MARCKS
Gene ID (NCBI) 4082
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP29966
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Myristoylated Alanine Rich C-Kinase Substrate (MARCKS) is a ubiquitous, highly conserved protein among vertebrates, which is essential for postnatal survival, and has been widely studied for its functions in the brain and nervous system. Being highly expressed in nervous tissue, particularly during early development but persisting in the adult, it plays numerous roles related to brain growth, neuronal migration, neurite outgrowth, neurotransmitter release, and synaptic plasticity. Protein kinase C (PKC) phosphorylates MARCKS, which converts MARCKS from a membrane-bound protein to a cytoplasmic protein. The phosphorylation site of MARCKS protein is called the effector domain (ED). Its structure is highly conserved. It can be combined with cell membrane, PKC, calcium/calmodulin-dependent kineses (CaMK) and F-actin. Studies have shown that increased membrane-bound, non-phosphorylated MARCKS might be conducive to the stabilization of synaptic morphology. Phosphorylated MARCKS protein (P-MARCKS) can regulate the stability of actin network and alter the synaptic structure. (PMID: 30655546, PMID: 30155805)

实验方案

Product Specific Protocols
WB protocol for Phospho-MARCKS (Ser167/170) antibody 29145-1-APDownload protocol
IF protocol for Phospho-MARCKS (Ser167/170) antibody 29145-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols