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Phospho-MCL1 (Thr163) Polyclonal antibody

Phospho-MCL1 (Thr163) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29560-1-AP

Print datasheet

Synonyms

EAT, Mcl 1, Mcl 1l, Mcl 1s Delta TM, MCL1, mcl1/EAT, MCL1L, MCL1S, TM

验证数据展示

  • WB analysis using 29560-1-AP

    WB analysis using 29560-1-AP

    Non-treated and MG132 treated HeLa cells were subjected to SDS PAGE followed by western blot with 29560-1-AP (Phospho-MCL1 (Thr163) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as the loading control.

经过测试的应用

Positive WB detected inMG132 treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy MCL1 Ready-To-Use IHC Kit. MCL1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29560-1-AP targets Phospho-MCL1 (Thr163) in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myeloid cell leukemia sequence 1 (BCL2-related)
Synonyms EAT, Mcl 1, Mcl 1l, Mcl 1s Delta TM, MCL1, mcl1/EAT, MCL1L, MCL1S, TM
Calculated Molecular Weight 350 aa, 37 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC017197
Gene Symbol MCL1
Gene ID (NCBI) 4170
RRIDAB_2935417
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ07820
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MCL1 is an anti-apoptotic member of the BCL-2 family originally isolated from the ML-1 human myeloid leukemia cell line. Similar to BCL2 and BCL2L1, MCL1 can interact with BAX and/or BAK1 to inhibit mitochondria-mediated apoptosis. Recent studies show that MCL1 is upregulated in numerous hematological and solid tumor malignancies. Therefore, MCL1 has been suggested as a potential new therapeutic target. MCL1 can be phosphorylated by several protein kinases which enables the recognition of MCL1 by its E3 ubiquitin-ligases TrCP or FBW7 (PMID: 33308268). MCL1 shows higher stability when phosphorylated on threonine 163 (PMID: 16543145).

实验方案

Product Specific Protocols
WB protocol for Phospho-MCL1 (Thr163) antibody 29560-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols