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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68047-1-Ig (same clone as 68047-1-PBS)

    WB analysis using 68047-1-Ig (same clone as 68047-1-PBS)

    Non-treated A431 cells and nocodazole treated A431 cells were subjected to SDS PAGE followed by western blot with 68047-1-Ig (Phospho-MEK1 (Ser298) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 68047-1-PBS in a different storage buffer formulation.

  • WB analysis using 68047-1-Ig (same clone as 68047-1-PBS)

    WB analysis using 68047-1-Ig (same clone as 68047-1-PBS)

    Non-treated HeLa cells and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 68047-1-Ig (Phospho-MEK1 (Ser298) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 68047-1-PBS in a different storage buffer formulation.

Phospho-MEK1 (Ser298) Monoclonal antibody, PBS Only

Phospho-MEK1 (Ser298) Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68047-1-PBS

产品说明书

CloneNo. 3F10G10

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, Indirect ELISA

别名

ERK activator kinase 1, MAP kinase kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1, MAPKK1, MEK 1, MEK1, MKK1, Phospho-MEK1 (Ser298), PRKMK1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MAP2K1/MEK1 Ready-To-Use IHC Kit. MAP2K1/MEK1 primary antibody included.

产品信息

68047-1-PBS targets Phospho-MEK1 (Ser298) in WB, Indirect ELISA applications and shows reactivity with Human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 Human
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 mitogen-activated protein kinase kinase 1
别名 ERK activator kinase 1, MAP kinase kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1, MAPKK1, MEK 1, MEK1, MKK1, Phospho-MEK1 (Ser298), PRKMK1
计算分子量 43 kDa
观测分子量 40-50 kDa
GenBank蛋白编号BC139729
基因名称 MEK1
Gene ID (NCBI) 5604
ENSEMBL Gene IDENSG00000169032
RRIDAB_2918789
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ02750
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAP2K1 encodes MAPK1, also known as MEK1. MEK1 variants can enhance MEK1 expression and ERK1 phosphorylation that together lead to continuous activation of MEK/ERK signaling pathway. MEK1 bind directly to ERK2 through a region in the N terminus of MEK. In addition, a proline-rich (PR) regulatory sequence in MEK is also involved in MEK-ERK association and signal propagation. The coupling between MEK1 and ERK2 is enhanced through phosphorylation on S298 in the MEK1 PR region, whereas phosphorylation on MEK1 T292 releases the complex. MEK1 T292 is a substrate of ERK2, but the site is also phosphorylated at a basal level when ERK2 is inhibited, suggesting several regulators of this site . Although the S298 site in MEK2 has been conserved, it lacks the T292 phosphorylation site, and it is not a substrate of PAK1. (PMID: 31972311, PMID: 17928366, PMID: 22177953)