Phospho-MEK1 (Thr292) Monoclonal antibody

Phospho-MEK1 (Thr292) Monoclonal Antibody for WB, ELISA, FC (Intra)
Cat No. 67873-1-Ig

产品说明书

CloneNo. 2D7A8

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, ELISA, FC (Intra)

MAP2K1, MEK1, MAPKK 1, MAP2K 1, EC:2.7.12.2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inNIH/3T3 cells, A431 cells, Calyculin A treated HeLa cells, Nocodazole treated A431 cells, Calyculin A treated NIH/3T3 cells, Calyculin A treated HSC-T6 cells
Positive FC (Intra) detected inCalyculin A treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy MAP2K1/MEK1 Ready-To-Use IHC Kit. MAP2K1/MEK1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67873-1-Ig targets Phospho-MEK1 (Thr292) in WB, ELISA, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, ELISA, FC (Intra) Application Description
文献引用应用WB
经测试反应性 Human, mouse, rat
文献引用反应性mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 mitogen-activated protein kinase kinase 1
别名 MAP2K1, MEK1, MAPKK 1, MAP2K 1, EC:2.7.12.2
计算分子量 43 kDa
观测分子量 40-50 kDa
GenBank蛋白编号BC139729
基因名称 MEK1
Gene ID (NCBI) 5604
ENSEMBL Gene IDENSG00000169032
RRIDAB_2918631
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ02750
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAP2K1 encodes MAPK1, also known as MEK1. MEK1 variants can enhance MEK1 expression and ERK1 phosphorylation that together lead to continuous activation of MEK/ERK signaling pathway. MEK1 bind directly to ERK2 through a region in the N terminus of MEK. In addition, a proline-rich (PR) regulatory sequence in MEK is also involved in MEK-ERK association and signal propagation. The coupling between MEK1 and ERK2 is enhanced through phosphorylation on S298 in the MEK1 PR region, whereas phosphorylation on MEK1 T292 releases the complex. MEK1 T292 is a substrate of ERK2, but the site is also phosphorylated at a basal level when ERK2 is inhibited, suggesting several regulators of this site . Although the S298 site in MEK2 has been conserved, it lacks the T292 phosphorylation site, and it is not a substrate of PAK1. (PMID: 31972311, PMID: 17928366, PMID: 22177953)

实验方案

Product Specific Protocols
WB protocol for Phospho-MEK1 (Thr292) antibody 67873-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse

J Ethnopharmacol

Classical prescription Floris Sophorae Powder treat colorectal cancer by regulating KRAS/MEK-ERK signaling pathway

Authors - Li-Ying Han
ratWB

J Ethnopharmacol

Jia-Wei-Si-Miao-Yong-An Fang stimulates the healing of acute radiation-induced cutaneous wounds through MAPK/ERK pathway

Authors - Yin Wang
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