Phospho-MEK1 (Thr386) Monoclonal antibody
Phospho-MEK1 (Thr386) Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1G6A2
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, A431 cells, NIH/3T3 cells, nocodazole treated HEK-293 cells, nocodazole treated A431 cells, Calyculin A treated NIH/3T3 cells, Calyculin A treated HeLa cells, λ phosphatase treated HeLa cells |
| Positive IF/ICC detected in | Calyculin A treated HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy MAP2K1/MEK1 Ready-To-Use IHC Kit. MAP2K1/MEK1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
68015-1-Ig targets Phospho-MEK1 (Thr386) in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | mitogen-activated protein kinase kinase 1 |
| Synonyms | MEK1 (Thr386), p MEK1 , p MEK1 (Thr386), p MEK1 Thr386, Phospho MEK1 |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 40-50 kDa |
| GenBank Accession Number | BC139729 |
| Gene Symbol | MEK1 |
| Gene ID (NCBI) | 5604 |
| ENSEMBL Gene ID | ENSG00000169032 |
| RRID | AB_2918761 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q02750 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAP2K1 encodes MAPK1, also known as MEK1. MEK1 variants can enhance MEK1 expression and ERK1 phosphorylation that together lead to continuous activation of MEK/ERK signaling pathway. MEK1 bind directly to ERK2 through a region in the N terminus of MEK. In addition, a proline-rich (PR) regulatory sequence in MEK is also involved in MEK-ERK association and signal propagation. The coupling between MEK1 and ERK2 is enhanced through phosphorylation on S298 in the MEK1 PR region, whereas phosphorylation on MEK1 T292 releases the complex. MEK1 T292 is a substrate of ERK2, but the site is also phosphorylated at a basal level when ERK2 is inhibited, suggesting several regulators of this site . Although the S298 site in MEK2 has been conserved, it lacks the T292 phosphorylation site, and it is not a substrate of PAK1. (PMID: 31972311, PMID: 17928366, PMID: 22177953)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-MEK1 (Thr386) antibody 68015-1-Ig | Download protocol |
| IF protocol for Phospho-MEK1 (Thr386) antibody 68015-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
