Phospho-MEK6 (Ser207) Polyclonal antibody
Phospho-MEK6 (Ser207) Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | UV treated HEK-293 cells, HEK-293 cells, HeLa cells, Anisomycin treated HeLa cells |
The antibody also detects the phosphorylated at Ser218 of the MEK3.
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:6000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
28903-1-AP targets Phospho-MEK6 (Ser207) in WB, ELISA applications and shows reactivity with Human samples.
Tested Applications | WB, ELISA Application Description |
Tested Reactivity | Human |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | mitogen-activated protein kinase kinase 6 |
Synonyms | MAP kinase kinase 6, MAP2K6, MAPK/ERK kinase 6, MAPKK 6, MAPKK6, MEK 6, MEK6, MKK6, Phospho-MAP2K6 (Ser207), Phospho-MEK3 (Ser218)/MEK6 (Ser207), Phospho-MEK6 (Ser207), PRKMK6, SAPKK3 |
Calculated Molecular Weight | 334 aa, 37 kDa |
Observed Molecular Weight | 35-37 kDa |
GenBank Accession Number | BC012009 |
Gene Symbol | MEK6 |
Gene ID (NCBI) | 5608 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P52564 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAPKK6 (Dual specificity mitogen activated protein kinase kinase 6), also known as MAP2K6 / MEK6 / MKK6, is a member of MAPKK protein kinase family. MKK6 plays an important role in intracellular signaling pathways leading toward activation of the p38 MAP kinase. MEK6 phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. Phosphorylation of Ser207 and Thr211 by MAP3Ks is critical for MEK6 activation, and acetylation of these resides by VopA blocks MEK6 activity(PMID: 26648936). The antibody also recognizes the ser218 phosphorylation site of MEK3.
实验方案
Product Specific Protocols | |
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WB protocol for Phospho-MEK6 (Ser207) antibody 28903-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |