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Phospho-Neurofibromin 2 (Ser518) Polyclonal antibody

Phospho-Neurofibromin 2 (Ser518) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28851-1-AP

Print datasheet

Synonyms

Phospho-NF2 (Ser518), NF2, Merlin, Moesin-ezrin-radixin-like protein, Neurofibromin 2, Neurofibromin-2

验证数据展示

  • WB analysis using 28851-1-AP

    WB analysis using 28851-1-AP

    Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 28851-1-AP (Phospho-NF2 (Ser518) antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

经过测试的应用

Positive WB detected inCalyculin A treated HEK-293T cells, Calyculin A treated PC-3 cells, HEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy NF2 Ready-To-Use IHC Kit. NF2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28851-1-AP targets Phospho-Neurofibromin 2 (Ser518) in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name neurofibromin 2 (merlin)
Synonyms Phospho-NF2 (Ser518), NF2, Merlin, Moesin-ezrin-radixin-like protein, Neurofibromin 2, Neurofibromin-2
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC007279
Gene Symbol NF2
Gene ID (NCBI) 4771
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35240
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neurofibromatosis 2 (NF2) tumor suppressor protein-merlin is involved in Hippo/SWH signaling pathway that associated with tumor suppression by restricting proliferation and promoting apoptosis. It has been reported that merlin can change to phosphorylation form which inactivates merlin tumor suppressive abilities at serine 518 induced by Rac effector p21-activated kinase 2. The mutation of the NF2 tumor suppressor gene may lead to nervous system tumors, mesotheliomas and metastatic tumors. 28851-1-AP antibody is specific to phosphorylation of NF2 at serine 518. (PMID: 11782491, 14724586, 18361411, 18835652, 32115406)

实验方案

Product Specific Protocols
WB protocol for Phospho-Neurofibromin 2 (Ser518) antibody 28851-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols