验证数据展示
经过测试的应用
| Positive WB detected in | VEGF treated HUVEC cells, EGF treated HUVEC cells, Cobalt Chloride treated HUVEC cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 6 publications below |
产品信息
28939-1-AP targets Phospho-eNOS (Thr495) in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nitric oxide synthase 3 (endothelial cell) |
| 别名 | NOS3, eNOS, EC-NOS, ECNOS, EC:1.14.13.39 |
| 计算分子量 | 133 kDa |
| 观测分子量 | 133 kDa |
| GenBank蛋白编号 | BC063294 |
| 基因名称 | eNOS |
| Gene ID (NCBI) | 4846 |
| RRID | AB_2881234 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P29474 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Endothelial NOS (eNOS), also known as nitric oxide synthase 3 (NOS3), has a protective function in the cardiovascular system, which is attributed to NO production. Polymorphisms in NOS3 gene affects the susceptibility to several diseases such as hypertension, preeclampsia, diabetes mellitus, obesity, erectile dysfunction, and migraine. The sequence homology of eNOS protein in human and mouse is above 90%, and phosphorylation of threonine at 495 site in human corresponds to 494 site in mouse.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-eNOS (Thr495) antibody 28939-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Hypertension Statins Prevent the Deleterious Consequences of Placental Chemerin Upregulation in Preeclampsia | ||
Commun Biol Targeting GPR39 in structure-based drug discovery reduces Ang II-induced hypertension | ||
J Ethnopharmacol Dahuang Zhechong pill attenuates hepatic sinusoidal capillarization in liver cirrhosis and hepatocellular carcinoma rat model via MK/integrin signaling pathway | ||
Drug Des Devel Ther Clopidogrel-Induced Gastric Injury in Rats is Attenuated by Stable Gastric Pentadecapeptide BPC 157. | ||
J Oncol Hax-1 Regulates Radiation-Induced Mitochondrial-Dependent Apoptosis of Uveal Melanoma Cells through PI3K/AKT/eNOS Pathway. | ||
Cytotherapy Porous gelatin microspheres implanted with adipose mesenchymal stromal cells promote angiogenesis via protein kinase B/endothelial nitric oxide synthase signaling pathway in bladder reconstruction |