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Phospho-eNOS (Thr495) Polyclonal antibody

Phospho-eNOS (Thr495) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28939-1-AP

Print datasheet

Synonyms

cNOS, Constitutive NOS, EC NOS, ECNOS, Endothelial NOS, eNOS, NOS type III, NOS3, NOSIII, Phospho-NOS3 (Thr495)

验证数据展示

  • WB analysis using 28939-1-AP

    WB analysis using 28939-1-AP

    Non-treated, VEGF (HZ-1038), EGF and Cobalt Chloride treated HUVEC cells were subjected to SDS PAGE followed by western blot with 28939-1-AP (Phospho-NOS3 (Thr495) antibody) at dilution of 1:1000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

经过测试的应用

Positive WB detected inVEGF treated HUVEC cells, EGF treated HUVEC cells, Cobalt Chloride treated HUVEC cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 5 publications below

产品信息

28939-1-AP targets Phospho-eNOS (Thr495) in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nitric oxide synthase 3 (endothelial cell)
Synonyms cNOS, Constitutive NOS, EC NOS, ECNOS, Endothelial NOS, eNOS, NOS type III, NOS3, NOSIII, Phospho-NOS3 (Thr495)
Calculated Molecular Weight133 kDa
Observed Molecular Weight133 kDa
GenBank Accession NumberBC063294
Gene Symbol eNOS
Gene ID (NCBI) 4846
RRIDAB_2881234
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP29474
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Endothelial NOS (eNOS), also known as nitric oxide synthase 3 (NOS3), has a protective function in the cardiovascular system, which is attributed to NO production. Polymorphisms in NOS3 gene affects the susceptibility to several diseases such as hypertension, preeclampsia, diabetes mellitus, obesity, erectile dysfunction, and migraine.

实验方案

Product Specific Protocols
WB protocol for Phospho-eNOS (Thr495) antibody 28939-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Hypertension

Statins Prevent the Deleterious Consequences of Placental Chemerin Upregulation in Preeclampsia

Authors - Lunbo Tan
View Article
ratWB

J Ethnopharmacol

Dahuang Zhechong pill attenuates hepatic sinusoidal capillarization in liver cirrhosis and hepatocellular carcinoma rat model via MK/integrin signaling pathway

Authors - Chuankui Fu
View Article
ratWB

Drug Des Devel Ther

Clopidogrel-Induced Gastric Injury in Rats is Attenuated by Stable Gastric Pentadecapeptide BPC 157.

Authors - Hailu Wu
View Article
humanWB

J Oncol

Hax-1 Regulates Radiation-Induced Mitochondrial-Dependent Apoptosis of Uveal Melanoma Cells through PI3K/AKT/eNOS Pathway.

Authors - Sha Wang
View Article
humanWB

Cytotherapy

Porous gelatin microspheres implanted with adipose mesenchymal stromal cells promote angiogenesis via protein kinase B/endothelial nitric oxide synthase signaling pathway in bladder reconstruction

Authors - Jun Zhao
View Article