Phospho-P53 (Ser15) Polyclonal antibody

Phospho-P53 (Ser15) Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 28961-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IF/ICC, ELISA and More (2)

TP53, P53, Tumour Protein p53, Tumor suppressor p53, tumor protein p53

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inetoposide treated HT-29 cells
Positive IF/ICC detected inetoposide treated HT-29 cells, HT-29 cells
Planning an IHC experiment? We recommend our IHCeasy P53 Ready-To-Use IHC Kit. P53 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28961-1-AP targets Phospho-P53 (Ser15) in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human
文献引用反应性human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tumor protein p53
别名 TP53, P53, Tumour Protein p53, Tumor suppressor p53, tumor protein p53
计算分子量 44 kDa
观测分子量53 kDa
GenBank蛋白编号BC003596
基因名称 P53
Gene ID (NCBI) 7157
RRIDAB_2881236
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP04637
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis . DNA damage induces phosphorylation of p53 at Ser15 and Ser20 and leads to a reduced interaction between p53 and its negative regulator, the oncoprotein MDM2. p53 can be phosphorylated by ATM, ATR, and DNA-PK at Ser15 and Ser37. Phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and activation of p53 in response to DNA damage.

实验方案

Product Specific Protocols
WB protocol for Phospho-P53 (Ser15) antibody 28961-1-APDownload protocol
IF protocol for Phospho-P53 (Ser15) antibody 28961-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Adv Sci (Weinh)

Transgelin Promotes Glioblastoma Stem Cell Hypoxic Responses and Maintenance Through p53 Acetylation

Authors - Huan Li
mouseWB

Aging Cell

Excessive processing and acetylation of OPA1 aggravate age-related hearing loss via the dysregulation of mitochondrial dynamics

Authors - Andi Zhang
mouseIF

Acta Pharmacol Sin

Exosomes are involved in total body irradiation-induced intestinal injury in mice.

Authors - Hang Li
humanWB

J Nutr Biochem

Lycopene suppresses gastric cancer cell growth without affecting normal gastric epithelial cells

Authors - Ying Zhou
humanWB

Clin Epigenetics

Comprehensive comparison between azacytidine and decitabine treatment in an acute myeloid leukemia cell line

Authors - Tina Aumer
humanWB

Apoptosis

High-dose copper activates p53-independent apoptosis through the induction of nucleolar stress in human cell lines.

Authors - Chieh-Hsin Chen
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