验证数据展示
经过测试的应用
Positive WB detected in | etoposide treated HT-29 cells |
Positive IF/ICC detected in | etoposide treated HT-29 cells, HT-29 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
28961-1-AP targets Phospho-P53 (Ser15) in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | human |
文献引用反应性 | human, mouse |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | tumor protein p53 |
别名 | TP53, P53, Tumour Protein p53, Tumor suppressor p53, tumor protein p53 |
计算分子量 | 44 kDa |
观测分子量 | 53 kDa |
GenBank蛋白编号 | BC003596 |
基因名称 | P53 |
Gene ID (NCBI) | 7157 |
RRID | AB_2881236 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P04637 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis . DNA damage induces phosphorylation of p53 at Ser15 and Ser20 and leads to a reduced interaction between p53 and its negative regulator, the oncoprotein MDM2. p53 can be phosphorylated by ATM, ATR, and DNA-PK at Ser15 and Ser37. Phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and activation of p53 in response to DNA damage.
实验方案
Product Specific Protocols | |
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WB protocol for Phospho-P53 (Ser15) antibody 28961-1-AP | Download protocol |
IF protocol for Phospho-P53 (Ser15) antibody 28961-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Adv Sci (Weinh) Transgelin Promotes Glioblastoma Stem Cell Hypoxic Responses and Maintenance Through p53 Acetylation | ||
Aging Cell Excessive processing and acetylation of OPA1 aggravate age-related hearing loss via the dysregulation of mitochondrial dynamics | ||
Acta Pharmacol Sin Exosomes are involved in total body irradiation-induced intestinal injury in mice. | ||
J Nutr Biochem Lycopene suppresses gastric cancer cell growth without affecting normal gastric epithelial cells | ||
Clin Epigenetics Comprehensive comparison between azacytidine and decitabine treatment in an acute myeloid leukemia cell line | ||
Apoptosis High-dose copper activates p53-independent apoptosis through the induction of nucleolar stress in human cell lines. |