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Phospho-P53 (Ser392) Polyclonal antibody

Phospho-P53 (Ser392) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28963-1-AP

Print datasheet

Synonyms

Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, P53, Phospho-P53 (Ser392), Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53

验证数据展示

  • WB analysis using 28963-1-AP

    WB analysis using 28963-1-AP

    Non-treated HEK-293, phosphatase inhibitor treated and λ phosphatase treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 28963-1-AP (Phospho-P53 (Ser392) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

经过测试的应用

Positive WB detected inλ phosphatase treated HEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy P53 Ready-To-Use IHC Kit. P53 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

28963-1-AP targets Phospho-P53 (Ser392) in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tumor protein p53
Synonyms Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, P53, Phospho-P53 (Ser392), Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC003596
Gene Symbol P53
Gene ID (NCBI) 7157
RRIDAB_2918224
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP04637
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

P53 is a 53 kDa protein that is activated in response to alteration of normal cell homeostasis, including DNA damage, nutrient starvation, heat shock, virus infection, pH change, hypoxia, and oncogene activation. P53 maintains genetic stability by regulating different processes, such as cell-cycle arrest, DNA synthesis and repair, programmed cell death, and energy metabolism. In non-stressed conditions these proteins bind p53, ubiquitylate it and target it for degradation by the proteasome. In stressed conditions the function of the MdM2-MdM4 complex is blocked by phosphorylation, protein-binding events and/or enhanced degradation. Phosphorylated at Ser-315 and Ser-392 by CDK2 in response to DNA-damage.(PMID: 19935675, PMID: 24379683)

实验方案

Product Specific Protocols
WB protocol for Phospho-P53 (Ser392) antibody 28963-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Chem Biol Interact

Hsa_circ_0005050 interacts with ILF3 and affects cell apoptosis and proliferation by disrupting the balance between p53 and p65

Authors - Jingwen Tan
View Article