验证数据展示
经过测试的应用
| Positive WB detected in | HT-29 cells, λ phosphatase treated HT-29 cells |
Planning an IHC experiment? We recommend our IHCeasy P53 Ready-To-Use IHC Kit. P53 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 8 publications below |
产品信息
28960-1-AP targets Phospho-P53 (Ser46) in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tumor protein p53 |
| 别名 | Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, P53, Phospho-P53 (Ser46), Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53 |
| 计算分子量 | 44 kDa |
| 观测分子量 | 53 kDa |
| GenBank蛋白编号 | BC003596 |
| 基因名称 | P53 |
| Gene ID (NCBI) | 7157 |
| RRID | AB_2918223 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P04637 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis. Phosphorylation of p53 at Ser46 regulates the ability of p53 to induce apoptosis. Homeodomain-interacting protein kinase 2 (HIPK2) Phosphorylates Ser46 off p53 and p38 can phosphorylate this site .
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-P53 (Ser46) antibody 28960-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Apoptosis High-dose copper activates p53-independent apoptosis through the induction of nucleolar stress in human cell lines. | ||
Exp Cell Res RPS14 promotes the development and progression of glioma via p53 signaling pathway | ||
Mol Cell Probes Long noncoding RNA RMRP ameliorates doxorubicin-induced apoptosis by interacting with PFN1 in a P53-Dependent manner | ||
Tissue Cell DEPDC1B enhances malignant phenotypes of multiple myeloma through upregulating CCNB1 and inhibiting p53 signaling pathway | ||
Cell Biol Int LRP8 promotes tumorigenesis in ovarian cancer through inhibiting p53 signaling | ||
Toxicology Exploring the Multifaceted Impact of Lanthanides on Physiological Pathways in Human Breast Cancer Cells |