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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis using 29583-1-AP (same clone as 29583-1-PBS)

    WB analysis using 29583-1-AP (same clone as 29583-1-PBS)

    10 % fetal bovine serum treated HeLa cells and Serum-free treated HeLa cells were subjected to SDS PAGE followed by western blot with 29583-1-AP (Phospho-PDH E1 Alpha (Ser300) antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 29583-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 29583-1-AP (same clone as 29583-1-PBS)

    IF Staining of HepG2 using 29583-1-AP (same clone as 29583-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated HepG2 cells using Phospho-PDH E1 Alpha (Ser300) antibody (29583-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 29583-1-PBS in a different storage buffer formulation.

Phospho-PDH E1 Alpha (Ser300) Polyclonal antibody, PBS Only

Phospho-PDH E1 Alpha (Ser300) Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 29583-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PDHA1 Ready-To-Use IHC Kit. PDHA1 primary antibody included.

产品信息

29583-1-PBS targets Phospho-PDH E1 Alpha (Ser300) in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pyruvate dehydrogenase (lipoamide) alpha 1
别名
计算分子量 43 kDa
观测分子量 40 kDa
GenBank蛋白编号BC002406
基因名称 PDHA1
Gene ID (NCBI) 5160
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP08559
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PDH E1 Alpha (PDHA1), as the major component of PDH, can be phosphorylated and inactivated by PDHK1. PDHA1 contains three serine residues (Ser232, Ser293, or Ser300) that can be reversibly phosphorylated by a dedicated family of four inhibitory pyruvate dehydrogenase kinases (PDHK1-4) and two reactivating phosphatases (PDP1, 2). Hypoxia induces the expression of PDHK1 and PDHK3 and hyperphosphorylates PDHA1. Phosphorylated PDHA1 inactivates the whole pyruvate dehydrogenase complex (PDC), reduces pyruvate entering into the tricarboxylic acid (TCA) cycle for oxidative phosphorylation, enhances the Warburg effect and promotes tumorigenesis. (PMID: 30993888, PMID: 34749809)