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Phospho-PDH E1 Alpha (Ser300) Recombinant antibody, PBS Only

Phospho-PDH E1 Alpha (Ser300) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E19

Cat no : 80572-1-PBS

Print datasheet

Synonyms

PDH E1α, PDH E1 Alpha, PDHA1, PDHA 1, PDHA

验证数据展示

  • WB analysis using 80572-1-RR (same clone as 80572-1-PBS)

    WB analysis using 80572-1-RR (same clone as 80572-1-PBS)

    Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 80572-1-RR (Phospho-PDH E1 Alpha (Ser300) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control. This data was developed using the same antibody clone with 80572-1-PBS in a different storage buffer formulation.

  • WB analysis of HSC-T6 using 80572-1-RR (same clone as 80572-1-PBS)

    WB analysis of HSC-T6 using 80572-1-RR (same clone as 80572-1-PBS)

    Non-treated and Calyculin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 80572-1-RR (Phospho-PDH E1 Alpha (Ser300) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control. This data was developed using the same antibody clone with 80572-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 80572-1-RR (same clone as 80572-1-PBS)

    IF Staining of HepG2 using 80572-1-RR (same clone as 80572-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated HepG2 cells using Phospho-PDH E1 Alpha (Ser300) antibody (80572-1-RR, Clone: 2E19 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80572-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PDHA1 Ready-To-Use IHC Kit. PDHA1 primary antibody included.

产品信息

80572-1-PBS targets Phospho-PDH E1 Alpha (Ser300) in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name pyruvate dehydrogenase (lipoamide) alpha 1
Synonyms PDH E1α, PDH E1 Alpha, PDHA1, PDHA 1, PDHA
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC002406
Gene Symbol PDHA1
Gene ID (NCBI) 5160
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08559
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PDH E1 Alpha (PDHA1), as the major component of PDH, can be phosphorylated and inactivated by PDHK1. PDHA1 contains three serine residues (Ser232, Ser293, or Ser300) that can be reversibly phosphorylated by a dedicated family of four inhibitory pyruvate dehydrogenase kinases (PDHK1-4) and two reactivating phosphatases (PDP1, 2). Hypoxia induces the expression of PDHK1 and PDHK3 and hyperphosphorylates PDHA1. Phosphorylated PDHA1 inactivates the whole pyruvate dehydrogenase complex (PDC), reduces pyruvate entering into the tricarboxylic acid (TCA) cycle for oxidative phosphorylation, enhances the Warburg effect and promotes tumorigenesis. (PMID: 30993888, PMID: 34749809)