Phospho-PDH E1 Alpha (Ser300) Recombinant antibody
Phospho-PDH E1 Alpha (Ser300) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
2E19
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HSC-T6 cells, NIH/3T3 cells, Calyculin A treated HeLa cells, Calyculin A treated HEK-293 cells, Calyculin A treated HSC-T6 cells, Calyculin A treated NIH/3T3 cells |
Positive IF/ICC detected in | λ phosphatase treated HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
80572-1-RR targets Phospho-PDH E1 Alpha (Ser300) in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IF/ICC, ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | pyruvate dehydrogenase (lipoamide) alpha 1 |
Synonyms | PDH E1α, PDH E1 Alpha, PDHA1, PDHA 1, PDHA |
Calculated Molecular Weight | 43 kDa |
Observed Molecular Weight | 40 kDa |
GenBank Accession Number | BC002406 |
Gene Symbol | PDHA1 |
Gene ID (NCBI) | 5160 |
RRID | AB_2918903 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P08559 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Phospho-PDH E1 Alpha (Ser300) antibody 80572-1-RR | Download protocol |
IF protocol for Phospho-PDH E1 Alpha (Ser300) antibody 80572-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |