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验证数据展示

  • WB analysis using 80572-1-RR

    WB analysis using 80572-1-RR

    Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 80572-1-RR (Phospho-PDH E1 Alpha (Ser300) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control.

  • WB analysis of HSC-T6 using 80572-1-RR

    WB analysis of HSC-T6 using 80572-1-RR

    Non-treated and Calyculin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 80572-1-RR (Phospho-PDH E1 Alpha (Ser300) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control.

  • IF Staining of HepG2 using 80572-1-RR

    IF Staining of HepG2 using 80572-1-RR

    Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated HepG2 cells using Phospho-PDH E1 Alpha (Ser300) antibody (80572-1-RR, Clone: 2E19 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Phospho-PDH E1 Alpha (Ser300) Recombinant antibody

Phospho-PDH E1 Alpha (Ser300) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 80572-1-RR

产品说明书

CloneNo. 2E19
引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA and More (1)

别名

p PDH E1 Alpha, p PDH E1 Alpha (Ser300), p PDH E1 Alpha Ser300, PDH E1 Alpha (Ser300), Phospho PDH E1 Alpha

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HSC-T6 cells, NIH/3T3 cells, Calyculin A treated HeLa cells, Calyculin A treated HEK-293 cells, Calyculin A treated HSC-T6 cells, Calyculin A treated NIH/3T3 cells
Positive IF/ICC detected inλ phosphatase treated HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PDHA1 Ready-To-Use IHC Kit. PDHA1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80572-1-RR targets Phospho-PDH E1 Alpha (Ser300) in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 pyruvate dehydrogenase (lipoamide) alpha 1
别名 p PDH E1 Alpha, p PDH E1 Alpha (Ser300), p PDH E1 Alpha Ser300, PDH E1 Alpha (Ser300), Phospho PDH E1 Alpha
计算分子量 43 kDa
观测分子量 40 kDa
GenBank蛋白编号BC002406
基因名称 PDHA1
Gene ID (NCBI) 5160
RRIDAB_2918903
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP08559
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDH E1 Alpha (PDHA1), as the major component of PDH, can be phosphorylated and inactivated by PDHK1. PDHA1 contains three serine residues (Ser232, Ser293, or Ser300) that can be reversibly phosphorylated by a dedicated family of four inhibitory pyruvate dehydrogenase kinases (PDHK1-4) and two reactivating phosphatases (PDP1, 2). Hypoxia induces the expression of PDHK1 and PDHK3 and hyperphosphorylates PDHA1. Phosphorylated PDHA1 inactivates the whole pyruvate dehydrogenase complex (PDC), reduces pyruvate entering into the tricarboxylic acid (TCA) cycle for oxidative phosphorylation, enhances the Warburg effect and promotes tumorigenesis. (PMID: 30993888, PMID: 34749809)

实验方案

Product Specific Protocols
WB protocol for Phospho-PDH E1 Alpha (Ser300) antibody 80572-1-RRDownload protocol
IF protocol for Phospho-PDH E1 Alpha (Ser300) antibody 80572-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB,IP

Science

Boosting neuronal activity-driven mitochondrial DNA transcription improves cognition in aged mice

Authors - Wenwen Li
View Article
human,mouseWB

Nat Commun

NOTCH1 mitochondria localization during heart development promotes mitochondrial metabolism and the endothelial-to-mesenchymal transition in mice

Authors - Jie Wang
View Article