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Phospho-PDPK1 (Ser241) Polyclonal antibody

Phospho-PDPK1 (Ser241) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 29241-1-AP

Print datasheet

Synonyms

hPDK1, PDK1, PDPK1, PRO0461

验证数据展示

  • WB analysis using 29241-1-AP

    WB analysis using 29241-1-AP

    Non-treated PC-3 and PDGF (HZ-1215) treated PC-3 cells were subjected to SDS PAGE followed by western blot with 29241-1-AP (Phospho-PDPK1 (Ser241) antibody) at dilution of 1:1000 incubated at 4℃ overnight.

  • WB analysis using 29241-1-AP

    WB analysis using 29241-1-AP

    Non-treated NIH/3T3 and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29241-1-AP (Phospho-PDPK1 (Ser241) antibody) at dilution of 1:2000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

经过测试的应用

Positive WB detected inPDGF treated PC-3 cells, PDGF treated NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy PDPK1 Ready-To-Use IHC Kit. PDPK1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

29241-1-AP targets Phospho-PDPK1 (Ser241) in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, mouse
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name 3-phosphoinositide dependent protein kinase-1
Synonyms hPDK1, PDK1, PDPK1, PRO0461
Calculated Molecular Weight 556 aa, 63 kDa
Observed Molecular Weight60-63 kDa
GenBank Accession NumberBC012103
Gene Symbol PDPK1
Gene ID (NCBI) 5170
RRIDAB_2918256
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15530
Storage Buffer PBS with 0.02% sodium azide, 50% glycerol pH 7.3 and 0.05% BSA
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

3-Phosphoinositide dependent protein kinase-1 (PDK1 or PDPK1) is a serine-threonine kinase belonging to AGC kinase family. PDK1 plays a critical role in establishing ACD (Asymmetric cell division) in the epithelium (PMID: 27184845). The kinase activity of PDK1 depends on phosphatidyl inositol 3-kinase (PI3K), a key intermediate in signaling pathways including those from growth factor receptors and adhesion molecules. Substrates of PDK1, including AKT and the protein kinase C (PKC) isozymes, regulate a number of essential cell functions (PMID: 20027184). PDPK1 has 5 isoforms produced by alternative splicing of 48~63 kDa and is detected as 60-63 kDa.

实验方案

Product Specific Protocols
WB protocol for Phospho-PDPK1 (Ser241) antibody 29241-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Rep

A p85 isoform switch enhances PI3K activation on endosomes by a MAP4- and PI3P-dependent mechanism

Authors - Narendra Thapa
View Article