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验证数据展示

  • WB analysis using 29241-1-AP

    WB analysis using 29241-1-AP

    Non-treated PC-3 and PDGF (HZ-1215) treated PC-3 cells were subjected to SDS PAGE followed by western blot with 29241-1-AP (Phospho-PDPK1 (Ser241) antibody) at dilution of 1:1000 incubated at 4℃ overnight.

  • WB analysis using 29241-1-AP

    WB analysis using 29241-1-AP

    Non-treated NIH/3T3 and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29241-1-AP (Phospho-PDPK1 (Ser241) antibody) at dilution of 1:2000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Phospho-PDPK1 (Ser241) Polyclonal antibody

Phospho-PDPK1 (Ser241) Polyclonal Antibody for WB, ELISA
Cat No. 29241-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse and More (1)

应用

WB, ELISA

别名

hPDK1, PDK1, PDPK1, PRO0461

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPDGF treated PC-3 cells, PDGF treated NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy PDPK1 Ready-To-Use IHC Kit. PDPK1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

29241-1-AP targets Phospho-PDPK1 (Ser241) in WB, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB
经测试反应性 Human, mouse
文献引用反应性human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 3-phosphoinositide dependent protein kinase-1
别名 hPDK1, PDK1, PDPK1, PRO0461
计算分子量 556 aa, 63 kDa
观测分子量60-63 kDa
GenBank蛋白编号BC012103
基因名称 PDPK1
Gene ID (NCBI) 5170
RRIDAB_2918256
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15530
储存缓冲液 PBS with 0.02% sodium azide, 50% glycerol pH 7.3 and 0.05% BSA
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

3-Phosphoinositide dependent protein kinase-1 (PDK1 or PDPK1) is a serine-threonine kinase belonging to AGC kinase family. PDK1 plays a critical role in establishing ACD (Asymmetric cell division) in the epithelium (PMID: 27184845). The kinase activity of PDK1 depends on phosphatidyl inositol 3-kinase (PI3K), a key intermediate in signaling pathways including those from growth factor receptors and adhesion molecules. Substrates of PDK1, including AKT and the protein kinase C (PKC) isozymes, regulate a number of essential cell functions (PMID: 20027184). PDPK1 has 5 isoforms produced by alternative splicing of 48~63 kDa and is detected as 60-63 kDa.

实验方案

Product Specific Protocols
WB protocol for Phospho-PDPK1 (Ser241) antibody 29241-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Rep

A p85 isoform switch enhances PI3K activation on endosomes by a MAP4- and PI3P-dependent mechanism

Authors - Narendra Thapa
View Article