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验证数据展示

  • WB analysis using 60597-1-Ig

    WB analysis using 60597-1-Ig

    Non-treated cells and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 60597-1-Ig (EIF2AK3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 60597-1-Ig

    WB analysis using 60597-1-Ig

    Non-treated PC-3 cells and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 60597-1-Ig (EIF2AK3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 60597-1-Ig

    WB analysis using 60597-1-Ig

    Non-treated NIH/3T3 cells and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60597-1-Ig (EIF2AK3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Phospho-PERK/EIF2AK3 (Ser719) Monoclonal antibody

Phospho-PERK/EIF2AK3 (Ser719) Monoclonal Antibody for WB, ELISA
Cat No. 60597-1-Ig

Print datasheet

CloneNo. 3H7A11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

EIF2AK3, PERK, HsPEK, Eukaryotic translation initiation factor 2-alpha kinase 3, EIF2AK 3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, NIH/3T3 cells, PC-3 cells, Jurkat cells, Calyculin A treated Jurkat cells, Calyculin A treated HEK-293 cells, Calyculin A treated PC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60597-1-Ig targets Phospho-PERK/EIF2AK3 (Ser719) in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name eukaryotic translation initiation factor 2-alpha kinase 3
Synonyms EIF2AK3, PERK, HsPEK, Eukaryotic translation initiation factor 2-alpha kinase 3, EIF2AK 3
Calculated Molecular Weight 1116 aa, 125 kDa
Observed Molecular Weight140 kDa
GenBank Accession NumberBC126354
Gene Symbol PERK
Gene ID (NCBI) 9451
RRIDAB_3670269
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9NZJ5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EIF2AK3 encodes the protein kinase RNA-like ER kinase (PERK), a key regulator of the unfolded protein response (UPR) in response to ER stress. Under ER stress conditions, activation of PERK is triggered by the dissociation of glucose-regulated protein (GRP) 78 (also known as BiP) from its luminal domain, followed by oligomerization and autophosphorylation. Phosphorylated PERK subsequently phosphorylates eukaryotic translation initiation factor 2 alpha (eif2α), to attenuate global protein translation and reduce incoming ER protein load via upregulated ER chaperone expression. (PMID: 35922637, PMID: 32029570)

实验方案

Product Specific Protocols
WB protocol for Phospho-PERK/EIF2AK3 (Ser719) antibody 60597-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols