Phospho-PKC Alpha (Ser657) Polyclonal antibody

Phospho-PKC Alpha (Ser657) Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 28926-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human and More (2)

应用

WB, IF/ICC, ELISA

AAG6, Phospho-PKC Alpha (Ser657), PKC A, PKC alpha, PKCA, PRKACA, PRKCA, Protein kinase C alpha type, protein kinase C, alpha

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inCalyculin A treated PC-3 cells
Positive IF/ICC detected inλ phosphatase treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy PRKCA Ready-To-Use IHC Kit. PRKCA primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

28926-1-AP targets Phospho-PKC Alpha (Ser657) in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 Human
文献引用反应性human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein kinase C, alpha
别名 AAG6, Phospho-PKC Alpha (Ser657), PKC A, PKC alpha, PKCA, PRKACA, PRKCA, Protein kinase C alpha type, protein kinase C, alpha
计算分子量 77 kDa
观测分子量77 kDa
GenBank蛋白编号AK055431
基因名称 PKC Alpha
Gene ID (NCBI) 5578
RRIDAB_2918214
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP17252
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PKCs are a family of serine/threonine kinases involved in various processes in cells including proliferation, differentiation, cell survival, and apoptosis. PKC family is composed of three different subgroups: conventional (cPKC), the novel (nPKC) and atypical (aPKC). PKCα, β1, β2, and γ belong to cPKC, PKCδ, ε, η and θ are nPKC, whereas aPKC comprises of PKCζ and λ/ι. All PKCs consist of the N-terminal regulatory region and C-terminal catalytic region (kinase domain). PKCs are physiologically activated by various extracellular signals transduced by hormones, growth factors, cytokines or antigens. The presence of activated PKCs on internal membranes leads to the phosphorylation of various interacting proteins. (PMID: 32466765, PMID: 12417016)

实验方案

Product Specific Protocols
WB protocol for Phospho-PKC Alpha (Ser657) antibody 28926-1-APDownload protocol
IF protocol for Phospho-PKC Alpha (Ser657) antibody 28926-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Dis

Neurokinin-1 receptor drives PKCɑ-AURKA/N-Myc signaling to facilitate the neuroendocrine progression of prostate cancer

Authors - Xiao-Wei Zhang
mouseWB

Appl Biochem Biotechnol

Sphincter of Oddi Dysfunction Induces Gallstone by Inhibiting the Expression of ABCB11 via PKC-α

Authors - Lichao Zhu
humanWB

Apoptosis

The resistance to anoikis, mediated by Spp1, and the evasion of immune surveillance facilitate the invasion and metastasis of hepatocellular carcinoma

Authors - Zhengwei Zhang
Loading...