验证数据展示
经过测试的应用
| Positive WB detected in | Calyculin A treated PC-3 cells |
| Positive IF/ICC detected in | λ phosphatase treated HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
28926-1-AP targets Phospho-PKC Alpha (Ser657) in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | protein kinase C, alpha |
| Synonyms | AAG6, Phospho-PKC Alpha (Ser657), PKC A, PKC alpha, PKCA, PRKACA, PRKCA, Protein kinase C alpha type, protein kinase C, alpha |
| Calculated Molecular Weight | 77 kDa |
| Observed Molecular Weight | 77 kDa |
| GenBank Accession Number | AK055431 |
| Gene Symbol | PKC Alpha |
| Gene ID (NCBI) | 5578 |
| RRID | AB_2918214 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P17252 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PKCs are a family of serine/threonine kinases involved in various processes in cells including proliferation, differentiation, cell survival, and apoptosis. PKC family is composed of three different subgroups: conventional (cPKC), the novel (nPKC) and atypical (aPKC). PKCα, β1, β2, and γ belong to cPKC, PKCδ, ε, η and θ are nPKC, whereas aPKC comprises of PKCζ and λ/ι. All PKCs consist of the N-terminal regulatory region and C-terminal catalytic region (kinase domain). PKCs are physiologically activated by various extracellular signals transduced by hormones, growth factors, cytokines or antigens. The presence of activated PKCs on internal membranes leads to the phosphorylation of various interacting proteins. (PMID: 32466765, PMID: 12417016)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-PKC Alpha (Ser657) antibody 28926-1-AP | Download protocol |
| IF protocol for Phospho-PKC Alpha (Ser657) antibody 28926-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Dis Neurokinin-1 receptor drives PKCɑ-AURKA/N-Myc signaling to facilitate the neuroendocrine progression of prostate cancer | ||
Appl Biochem Biotechnol Sphincter of Oddi Dysfunction Induces Gallstone by Inhibiting the Expression of ABCB11 via PKC-α | ||
Apoptosis The resistance to anoikis, mediated by Spp1, and the evasion of immune surveillance facilitate the invasion and metastasis of hepatocellular carcinoma |